pCMV-FLAG-C-TMEM206-4

Catalog number

PVT23474
Price

Please ask
Size

Gentaur supplies 2ug of lyophilized CMV-FLAG-C-TMEM206-4 Plasmid in powder form at 4°C to be centrifuged at 5000rpm/min for 1min. Add 20μl ddH2O in to the vial of the pCMV-FLAG-C-TMEM206-4 plasmid.

Vectro Map

The pCMV promotor is combined with a FLAG vector sequence and a C gene in the plasmid vector map.
Properties

An anti-flag tag (FLAG fusion protein) is use to detect a FLAG-tag, or FLAG octapeptide, or FLAG epitope that is a polypeptide protein tag that can be added to a protein using recombinant DNA. This FLAG-tags have the sequence DYKDDDDK motiv. These tags are very useful to do protein purification by affinity chromatography. Also separation of recombinant, overexpressed proteins from cell lysates is done by FLAG go HIS tags. FLAGS are also used in the isolation of protein complexes with multiple subunits, because its mild purification procedure tends not to disrupt such complexes. It has been used to enrich proteins of height purity and quality to see the 3D crystal structure with x-ray. Suitable for in vivo use in cells. For electrophorese protein detection rabbit polyclonals anti Flag conjugation are the most suited antibodies.
Conjugation

Flag

Identity

HGNC:5560
Gene

IGHV1-38-4
Long gene name

immunoglobulin heavy variable 1-38-4 (non-functional)
Synonyms gene
- IGHV1-C
Synonyms gene name
- immunoglobulin heavy variable 1-C (provisional)
- immunoglobulin heavy variable 1-C (provisional, non-functional)
Synonyms
- IGHV1-c
GenBank acession
- KF698736
Locus

14q32.33 not on reference assembly
Discovery year

2000-04-04
Entrez gene record

28460
Classification
- Immunoglobulin heavy locus at 14q32.33

Identity

HGNC:14013
Gene

SMC4
Long gene name

structural maintenance of chromosomes 4
Synonyms gene
- SMC4L1
Synonyms gene name
- SMC4 (structural maintenance of chromosomes 4, yeast)-like 1
- SMC4 structural maintenance of chromosomes 4-like 1 (yeast)
Synonyms
- hCAP-C
- CAP-C
GenBank acession
- AF092564
Locus

3q25.33
Discovery year

2001-02-22
Entrez gene record

10051
Pubmed identfication
- 9789013
- 10319587
Classification
- Structural maintenance of chromosomes proteins
- Condensin I subunits
- Condensin II subunits
- MicroRNA protein coding host genes
VEGA ID

OTTHUMG00000159006

Identity

HGNC:25593
Gene

PACC1
Long gene name

proton activated chloride channel 1
Synonyms gene
- C1orf75
- TMEM206
Synonyms gene name
- chromosome 1 open reading frame 75
- transmembrane protein 206
Synonyms
- FLJ10874
- PAC
GenBank acession
- AK024066
Locus

1q32.3
Discovery year

2005-05-19
Entrez gene record

55248
Pubmed identfication
- 30417481
- 31318332
RefSeq identity
- NM_018252
VEGA ID

OTTHUMG00000036751