pcDNA3.1(+)-SGK2-Flag Plasmid
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Catalog number
PVTB01048-2a
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Price
383.73 USD
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Size
2 ug
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Properties
An anti-flag tag (FLAG fusion protein) is use to detect a FLAG-tag, or FLAG octapeptide, or FLAG epitope that is a polypeptide protein tag that can be added to a protein using recombinant DNA. This FLAG-tags have the sequence DYKDDDDK motiv. These tags are very useful to do protein purification by affinity chromatography. Also separation of recombinant, overexpressed proteins from cell lysates is done by FLAG go HIS tags. FLAGS are also used in the isolation of protein complexes with multiple subunits, because its mild purification procedure tends not to disrupt such complexes. It has been used to enrich proteins of height purity and quality to see the 3D crystal structure with x-ray. Suitable for in vivo use in cells. For electrophorese protein detection rabbit polyclonals anti Flag conjugation are the most suited antibodies.
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Conjugation
Flag
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Kit
Plasmid mini made and maxi DNA purification kits can be silica gel or anion exchange, endotoxin free and are used to produce pure plasmids that are small DNA molecules within a cell separated from chromosomal DNA and can replicate independently. They are most commonly found in bacteria as small circular, double-stranded DNA molecules; however, plasmids are sometimes present in archaea and eukaryotic organisms. In nature, plasmids often carry genes that may benefit the survival of the organism, for example antibiotic resistance. While the chromosomes are big and contain all the essential information for living, plasmids usually are very small and contain only additional information. Artificial plasmids are widely used as vectors in molecular cloning, serving to drive the replication of recombinant DNA sequences within host organisms.
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Gene target
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Gene symbol
SGK2, SGK3
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Short name
pcDNA3 1(+)-SGK2- Plasmid
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Technique
plasmid, plasmids in 1
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Label
Flag
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Alternative name
pcDNA3.1(+)-serum/glucocorticoid regulated kinase 2-Flag Plasmid
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Alternative technique
plasmids
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Alternative to gene target
serum/glucocorticoid regulated kinase 2, dJ138B7.2 and H-SGK2, SGK2 and IDBG-75934 and ENSG00000101049 and 10110, transferase activity, nuclei, Sgk2 and IDBG-211810 and ENSMUSG00000017868 and 27219, BT.32803 and IDBG-642748 and ENSBTAG00000021033 and 517909
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Gene info
Gene info
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