pCDNA3.1-FLAG-HIF1A

• Catalog number
  PVT20231
• Price
  Please ask
• Size
  Gentaur supplies 2ug of lyophilized CDNA3.1-FLAG-HIF1A Plasmid in powder form at 4°C to be centrifuged at 5000rpm/min for 1min. Add 20μl ddH2O in to the vial of the pCDNA3.1-FLAG-HIF1A plasmid.

• Vectro Map
  The pCDNA3.1 promotor is combined with a FLAG vector sequence and a HIF1A gene in the plasmid vector map.
• Properties
  An anti-flag tag (FLAG fusion protein) is use to detect a FLAG-tag, or FLAG octapeptide, or FLAG epitope that is a polypeptide protein tag that can be added to a protein using recombinant DNA. This FLAG-tags have the sequence DYKDDDDK motiv.  These tags are very useful to do protein purification by affinity chromatography. Also separation of recombinant, overexpressed proteins from cell lysates is done by FLAG go HIS tags. FLAGS are also used in the isolation of protein complexes with multiple subunits, because its mild purification procedure tends not to disrupt such complexes. It has been used to enrich proteins of height purity and quality to see the 3D crystal structure with x-ray. Suitable for in vivo use in cells. For electrophorese protein detection rabbit polyclonals anti Flag conjugation are the most suited antibodies.
• Conjugation
  Flag

• Gene target
  pCDNA3   HIF1A  
• Gene symbol
  HIF1A-AS3, HIF1A-AS1, HIF1A-AS2, HIF1A
• Short name
  pCDNA3 1- -HIF1A
• Label
  Flag
• Alternative name
  pCDNA3.1-FLAG-hypoxia inducible factor 1, alpha subunit (basic helix-loop-helix transcription factor)
• Alternative to gene target
  hypoxia inducible factor 1, alpha subunit (basic helix-loop-helix transcription factor), bHLHe78 and HIF-1A and HIF-1alpha and HIF1 and HIF1-ALPHA and MOP1 and PASD8, HIF1A and IDBG-8510 and ENSG00000100644 and 3091, Hsp90 protein binding, nuclei, Hif1a and IDBG-148620 and ENSMUSG00000021109 and 15251, HIF1A and IDBG-646757 and ENSBTAG00000020935 and 281814

Gene info

• Identity
  HGNC:54284
• Gene
  HIF1A-AS3
• Long gene name
  HIF1A antisense RNA 3
• Locus
  14q23.2
• Discovery year
  2019-05-01
• Entrez gene record
  105370526
• RefSeq identity
  • NR_144368
• Classification
  • Antisense RNAs

Gene info

• Identity
  HGNC:43014
• Gene
  HIF1A-AS1
• Long gene name
  HIF1A antisense RNA 1
• Synonyms gene name
  • HIF1A antisense RNA 1 (non-protein coding)
• Synonyms
  • 5'aHIF-1A
• GenBank acession
  • CN270307
• Locus
  14q23.2
• Discovery year
  2011-09-16
• Entrez gene record
  100750246
• Pubmed identfication
  • 21897117
  • 24875884
• RefSeq identity
  • NR_047116
• Classification
  • Antisense RNAs
• VEGA ID
  OTTHUMG00000171134

Gene info

• Identity
  HGNC:43015
• Gene
  HIF1A-AS2
• Long gene name
  HIF1A antisense RNA 2
• Synonyms gene name
  • HIF1A antisense RNA 2 (non-protein coding)
• Synonyms
  • 3'aHIF-1A
  • aHIF
• Locus
  14q23.2
• Discovery year
  2011-09-16
• Entrez gene record
  100750247
• Pubmed identfication
  • 21897117
  • 27018306
• RefSeq identity
  • NR_045406
• Classification
  • Antisense RNAs
• VEGA ID
  OTTHUMG00000171135

Gene info

• Identity
  HGNC:4910
• Gene
  HIF1A
• Long gene name
  hypoxia inducible factor 1 subunit alpha
• Synonyms gene name
  • hypoxia inducible factor 1, alpha subunit (basic helix-loop-helix transcription factor)
• Synonyms
  • MOP1
  • HIF-1alpha
  • PASD8
  • HIF1
  • bHLHe78
• Synonyms name
  • PAS domain-containing protein 8”, “Class E basic helix-loop-helix protein 78”, “Member of PAS protein 1
• GenBank acession
  • U22431
• Locus
  14q23.2
• Discovery year
  1995-03-27
• Entrez gene record
  3091
• Pubmed identfication
  • 8786149
  • 9079689
• RefSeq identity
  • NM_001530
• Classification
  • Basic helix-loop-helix proteins
• VEGA ID
  OTTHUMG00000140344

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