pCDNA3.1-FLAG-FTO

• Catalog number
  PVT19818
• Price
  Please ask
• Size
  Gentaur supplies 2ug of lyophilized CDNA3.1-FLAG-FTO Plasmid in powder form at 4°C to be centrifuged at 5000rpm/min for 1min. Add 20μl ddH2O in to the vial of the pCDNA3.1-FLAG-FTO plasmid.

• Vectro Map
  The pCDNA3.1 promotor is combined with a FLAG vector sequence and a FTO gene in the plasmid vector map.
• Properties
  An anti-flag tag (FLAG fusion protein) is use to detect a FLAG-tag, or FLAG octapeptide, or FLAG epitope that is a polypeptide protein tag that can be added to a protein using recombinant DNA. This FLAG-tags have the sequence DYKDDDDK motiv.  These tags are very useful to do protein purification by affinity chromatography. Also separation of recombinant, overexpressed proteins from cell lysates is done by FLAG go HIS tags. FLAGS are also used in the isolation of protein complexes with multiple subunits, because its mild purification procedure tends not to disrupt such complexes. It has been used to enrich proteins of height purity and quality to see the 3D crystal structure with x-ray. Suitable for in vivo use in cells. For electrophorese protein detection rabbit polyclonals anti Flag conjugation are the most suited antibodies.
• Conjugation
  Flag

• Gene target
  pCDNA3   FTO  
• Gene symbol
  FTO-IT1, FTO
• Short name
  pCDNA3 1- -FTO
• Label
  Flag
• Alternative name
  pCDNA3.1-FLAG-fat mass and obesity associated
• Alternative to gene target
  fat mass and obesity associated, FTO and IDBG-31147 and ENSG00000140718 and 79068, DNA-N1-methyladenine dioxygenase activity, nuclei, Fto and IDBG-179938 and ENSMUSG00000055932 and 26383, FTO and IDBG-631892 and ENSBTAG00000012501 and 618622

Gene info

• Identity
  HGNC:43758
• Gene
  FTO-IT1
• Long gene name
  FTO intronic transcript 1
• Synonyms gene name
  • FTO intronic transcript 1 (non-protein coding)
• GenBank acession
  • CA450585
• Locus
  16q12.2
• Discovery year
  2012-02-22
• Entrez gene record
  100505692
• RefSeq identity
  • NR_103838
• Classification
  • Intronic transcripts

Gene info

• Identity
  HGNC:24678
• Gene
  FTO
• Long gene name
  FTO alpha-ketoglutarate dependent dioxygenase
• Synonyms gene name
  • fat mass and obesity associated
  • FTO, alpha-ketoglutarate dependent dioxygenase
• Synonyms
  • KIAA1752
  • MGC5149
  • ALKBH9
• Synonyms name
  • alkB homolog 9
  • alpha-ketoglutarate-dependent dioxygenase
• GenBank acession
  • BC003583
• Locus
  16q12.2
• Discovery year
  2007-04-12
• Entrez gene record
  79068
• Pubmed identfication
  • 17434869
  • 17991826
  • 22002720
• RefSeq identity
  • NM_001080432
• Classification
  • Alkylation repair homologs
• VEGA ID
  OTTHUMG00000158780

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