pCAGGS-FLAG-CHUK

Quantity:

Properties

An anti-flag tag (FLAG fusion protein) is use to detect a FLAG-tag, or FLAG octapeptide, or FLAG epitope that is a polypeptide protein tag that can be added to a protein using recombinant DNA. This FLAG-tags have the sequence DYKDDDDK motiv. These tags are very useful to do protein purification by affinity chromatography. Also separation of recombinant, overexpressed proteins from cell lysates is done by FLAG go HIS tags. FLAGS are also used in the isolation of protein complexes with multiple subunits, because its mild purification procedure tends not to disrupt such complexes. It has been used to enrich proteins of height purity and quality to see the 3D crystal structure with x-ray. Suitable for in vivo use in cells. For electrophorese protein detection rabbit polyclonals anti Flag conjugation are the most suited antibodies.
Conjugation

Flag

Gene target

pCAGGS CHUK
Gene symbol

CHUK-DT, CHUK
Short name

pCAGGS- -CHUK
Label

Flag
Alternative name

pCAGGS-FLAG-conserved helix-loop-helix ubiquitous kinase
Alternative to gene target

conserved helix-loop-helix ubiquitous kinase, IKBKA and IKK-alpha and IKK1 and IKKA and NFKBIKA and TCF16, CHUK and IDBG-243385 and ENSG00000213341 and 1147, transferase activity, nuclei, Chuk and IDBG-169132 and ENSMUSG00000025199 and 12675, CHUK and IDBG-638708 and ENSBTAG00000007591 and 281073

Quantity:

Identity

HGNC:1974
Gene

CHUK
Long gene name

component of inhibitor of nuclear factor kappa B kinase complex
Synonyms gene
- TCF16
Synonyms gene name
- conserved helix-loop-helix ubiquitous kinase
Synonyms
- IKK1
- IKK-alpha
- IkBKA
- NFKBIKA
- IKKA
Synonyms name
- inhibitor of nuclear factor kappa-B kinase subunit alpha
- I-kappa-B kinase
GenBank acession
- AF009225
Locus

10q24.31
Discovery year

1995-03-15
Entrez gene record

1147
Pubmed identfication
- 7558004
- 16902410
RefSeq identity
- NM_001278
Classification
- I kappa B kinases
- IKK complex
VEGA ID

OTTHUMG00000018899