Lenti-(BB)-hPGK-KRAB-dCas9-P2A-BlastR
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Vectro Map
The Lenti promotor is combined with a (BB) vector sequence and a hPGK gene in the plasmid vector map.
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Description
Lenti kit or vector with CMV or EF promotors for producing Lentivirus (lenti-, Latin for "slow") or lentiviral particles in 293t cells. Lentiviruses are characterized by a long incubation period and often Puro selected. Lentiviruses can deliver a significant amount of viral RNA into the DNA of the host cell and have the unique ability among retroviruses of being able to infect non-dividing cells. They are one of the most efficient methods of a gene delivery vector. The are packaged in HEK 293T cells to get the highest possible lentiviral titers of 108 IFU/ml. The Luc reporter is the expression of the firefly luciferase gene. Puro stands for puromicin selection.
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Gene target
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Short name
-(BB)-hPGK-KRAB-dCas9-P2A-BlastR
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Technique
lenti, Lentivectors and viral particles
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Alternative name
lentiviral-(BB)-hPGK-KRAB-dCas9-P2A-BlastR
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Virus
lenti
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MeSH Data
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