Leukemia MLL-AF4 Fusion Gene Real Time RT-PCR Kit

Product type

Tumor
Instrument 1

Ⅰ, Ⅱ
Instrument 2

(Ⅰ) LightCycler 1.0 (Internal control can't be used for this system)(Ⅱ) LightCycler 2.0
Nucleic Acid

RNA
Note

CE Marked
Description

Fusion proteins or chimeric proteins are proteins created through the joining of two or more genes that originally coded for separate proteins. A GFP gene is often used as tag to a reporter gene. Fusion lentiverctors can be used as viral particles to produce proteins that carry for example a GFP tag. Antigen purification of recombinant fusion tag proteins is a frequent strategy using a Fralg tag. cDNA genes are a locus (or region) of DNA for functional transcript RNA or protein. An ELISA is used to detect the expressed protein in biological fluids, serum, saliva.
Group

PCR, polymerase chain reaction, RT-PCR mixes
About

TAQ or Pfu or Pfx or other enzymes are used for polycmerase chain reaction and have different specificity. The mores specific the lower the yield.
Properties

Thermocyclers can be callibrated for identical ramping curves to obtain a more accurate PCR.

Gene target

Leukemia MLL-AF4 Gene Real Time Kit
Gene symbol

AFF1, KMT2A
Short name

Leukemia MLL-AF4 Real Time RT-PCR Kit
Technique

RT-PCR, PCR, The polymerase chain reaction (PCR) amplifies the DNA in your sample. For real time PCR the cycle threshold Ct values willneed to be set before the experiment. Than the RT-PCR starts from RNA and real time PCR quantitates the cDNA so the RNA in the sample on given time of the experiment.
Alternative name

Leukemia myeloid/lymphoid and mixed-lineage leukemia (trithorax homolog, Drosophila)-AF4 Fusion Gene Real Time Reverse transcription PCR test kit reagent
Alternative technique

rtpcrkits, kits, dna-amplification
Alternative to gene target

v-kit Hardy-Zuckerman 4 feline sarcoma viral oncogene homolog, C-Kit and CD117 and PBT and SCFR, KIT and IDBG-18980 and ENSG00000157404 and 3815, transferase activity, Extracellular, Kit and IDBG-172083 and ENSMUSG00000005672 and 16590, KIT and IDBG-642326 and ENSBTAG00000002699 and 280832
Virus

leukemia

Identity

HGNC:7135
Gene

AFF1
Long gene name

AF4/FMR2 family member 1
Synonyms gene
- PBM1
- MLLT2
Synonyms gene name
- myeloid/lymphoid or mixed-lineage leukemia (trithorax (Drosophila) homolog); translocated to, 2
- pre-B-cell monocytic leukemia partner 1
- AF4/FMR2 family, member 1
Synonyms
- AF-4
- AF4
GenBank acession
- L22179
Locus

4q21.3-q22.1
Discovery year

2005-06-27
Entrez gene record

4299
Pubmed identfication
- 7689231
- 1423625
- 8353274
RefSeq identity
- NM_005935
Classification
- Super elongation complex
- AF4/FMR2 family
VEGA ID

OTTHUMG00000130603

Identity

HGNC:7132
Gene

KMT2A
Long gene name

lysine methyltransferase 2A
Synonyms gene
- MLL
Synonyms gene name
- myeloid/lymphoid or mixed-lineage leukemia (trithorax (Drosophila) homolog)
- myeloid/lymphoid or mixed-lineage leukemia (trithorax homolog, Drosophila)
- lysine (K)-specific methyltransferase 2A
Synonyms
- TRX1
- HRX
- ALL-1
- HTRX1
- CXXC7
- MLL1A
Synonyms name
- Histone-lysine N-methyltransferase 2A
GenBank acession
- L04284
Locus

11q23.3
Discovery year

1991-11-15
Entrez gene record

4297
Pubmed identfication
- 1720549
RefSeq identity
- NM_005933
Classification
- PHD finger proteins
- SET domain containing
- Lysine methyltransferases
- Zinc fingers CXXC-type
- Bromodomain containing
VEGA ID

OTTHUMG00000166337
Locus Specific Databases
- LRG_613

Name

Nucleic Acid Amplification Techniques
Concept

Scope note: Laboratory techniques that involve the in-vitro synthesis of many copies of DNA or RNA from one original template.
Tree numbers
- E05.393.620
Qualifiers

ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data