Perpetual Taq DNA Polymerase, Monoclonal antibody automatic “hot start” PCR system (2.5 u/µl).
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Catalog number
E2700-01
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Price
Please ask
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Size
2X200 u
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Short description
NA
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Properties
If you buy Antibodies supplied by EURX they should be stored frozen at - 24°C for long term storage and for short term at + 5°C. Thermocyclers can be callibrated for identical ramping curves to obtain a more accurate PCR. Hot start taq polymerases can be supplied as mix with dNTPs or Mg+ free Polymerases. The units are redifined for reactions but you can delute more this Hot start TAQ polymerase. Storage at -25 º C.
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About
Monoclonals of this antigen are available in different clones. Each murine monoclonal anibody has his own affinity specific for the clone. Mouse monoclonal antibodies are purified protein A or G and can be conjugated to FITC for flow cytometry or FACS and can be of different isotypes. TAQ or Pfu or Pfx or other enzymes are used for polycmerase chain reaction and have different specificity. The mores specific the lower the yield.
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Group
PCR, polymerase chain reaction
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French translation
anticorps
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Gene target
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Gene symbol
PRKDC, HLA-DOA
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Short name
Perpetual Taq DNA Polymerase, Monoclonal antibody automatic “hot start” PCR system (2 5 u/µl).
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Technique
Antibody, dna, PCR, antibodies against human proteins, antibodies for, Monoclonals or monoclonal antibodies, The polymerase chain reaction (PCR) amplifies the DNA in your sample. For real time PCR the cycle threshold Ct values willneed to be set before the experiment. Than the RT-PCR starts from RNA and real time PCR quantitates the cDNA so the RNA in the sample on given time of the experiment. Thermus Aquaticus or TAQ polymerase is a very robust enzyme that lacks endomuclease proof-reading. EURX TAQ has a far higher yield that other polymerases. TAQ is perfect for verificative PCR in an agarose gel. The bands in which positive primers and a negative control are included need to be compared to a DNA 100 bp molecular weight ladder. For proofreading mixes with pfu are required. It is supplied in 1
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Alternative name
Perpetual Taq Desoxyribonucleic acid polymerizing enzyme, monoclonal (antibody to-) automatic “hot start” PCR test kit system (2.5 u/µl).
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Alternative technique
antibodies, dna-amplification
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Gene info
Gene info
MeSH Data
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Name
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Concept
Scope note:
In vitro method for producing large amounts of specific DNA or RNA fragments of defined length and sequence from small amounts of short oligonucleotide flanking sequences (primers). The essential steps include thermal denaturation of the double-stranded target molecules, annealing of the primers to their complementary sequences, and extension of the annealed primers by enzymatic synthesis with DNA polymerase. The reaction is efficient, specific, and extremely sensitive. Uses for the reaction include disease diagnosis, detection of difficult-to-isolate pathogens, mutation analysis, genetic testing, DNA sequencing, and analyzing evolutionary relationships.
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Tree numbers
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Qualifiers
ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data
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