Biotin Conjugated Protein G
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Catalog number
BA1026-1
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Price
Please ask
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Size
1000 µl
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Type of the antibody protein
Protein G
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Raised in synthesized
synthesized polypeptide
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Conjugation
Biotin
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Product configuration
Solution with concentration 1mg/ml
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Immunogen
N/A
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Clonality
Protein G
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Analyses
IHC-P,IHC-F,ICC,WB,ELISA
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Storage conditions
Keep the Biotin Conjugated Protein G at plus four degrees Celsius for 12 months.
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Product details
Protein G is widely used as an affinity-based ligand for the purification of IgG, it can bind to all IgG subfamily from human, mouse and rat. Streptococcal protein G also has been used extensively for the purification of antibodies using the interaction of the Fc region with protein G. Many antibodies interact with protein G through a low-affinity binding site for the Fab region.
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Tips
The Biotin Conjugated Protein G is intended to be used for research analyses and it is not applicale for in vitro diagnostics.
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Properties
Biotin conjugates can be detected by horseradish peroxidase, alkaline phosphatase substrates or anti biotin conjugated antibodies. Avidin and Streptavidin bind to the small biotin and are couple to HRP or AP for ELISA. To break the streptavidin Biotin bond we suggest to use a 6 molar guanidine HCl solution with acidity of pH 1.6.
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Additional conjugation
Biotinylated
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