Biotin Conjugated Protein A

Type of the antibody protein

Protein A
Raised in synthesized

synthesized polypeptide
Conjugation

Biotin
Product configuration

Solution with concentration 1mg/ml
Immunogen

N/A
Clonality

Protein A
Analyses

IHC-P,IHC-F,ICC,WB,ELISA
Storage conditions

Keep the Biotin Conjugated Protein A at plus four degrees Celsius for 12 months.
Product details

Protein A is the component of the cytoderm of staphylococcus aureus. It was regarded as a kind of broad spectrum secondary antibody, because it can bind Fc fragments of a majority of immunoglobulin. It can react with guinea pig, rabbit globulin and human IgG1, 2, 4 strongly and react with mouse, bovine globulin and human IgA, E, M weakly.
Tips

The Biotin Conjugated Protein A is intended to be used for research analyses and it is not applicale for in vitro diagnostics.
Properties

Biotin conjugates can be detected by horseradish peroxidase, alkaline phosphatase substrates or anti biotin conjugated antibodies. Avidin and Streptavidin bind to the small biotin and are couple to HRP or AP for ELISA. To break the streptavidin Biotin bond we suggest to use a 6 molar guanidine HCl solution with acidity of pH 1.6.
Additional conjugation

Biotinylated