Beta Galactosidase (β-Gal) Inhibitor Screening Kit (Fluorometric)

• Catalog number
  K827-100
• Price
  Please ask
• Size
  100 assays

• Description
  Market's first and only kit for screening beta galactosidase Inhibitors. Simple, HTP compatible, 30 minutes protocol.
• Summary
  A simple and high-throughput adaptable assay kit used to fluorometrically (Ex/Em = 480/520 nm) screen/study/characterize potential inhibitors of beta galactosidase within 30 min.
• Detection Method
  Fluorescence (Ex/Em = 480/520 nm)
• Species Reactivity
  N/A
• Applications
  • Screening/characterizing/studying potential inhibitors of Beta-Galactosidase
• Sample Type
  Variable: Inhibitors, small molecules, proteins, etc.
• Features Benefits
  Simple and High throughput adaptable 30 minutes short protocol Detect using a fluorometer or fluorescence microplate reader.
• Storage Conditions
  -20°C
• Shipping Conditions
  gel pack
• Shelf life
  12 months
• Background
  Beta Galactosidase (β-Gal, EC: 3.2.1.23) is an enzyme which hydrolyzes the β-galactosides into monosacchanides. β-Gal is widely used as a reporter gene in the field of molecular biology. Senescence Associated β-Gal (SA-β-Gal) is an isoform of β-Gal which has the optimal activity at pH 6.0, and is mostly used as a biomarker for senescent cells (K802). β-Gal is an essential enzyme in humans and its deficiency results in Morquio’s Syndrome, a severe birth defect. β-Gal can also be used as a tool to study protein-protein interaction. In BioVision’s Beta-Galactocidase Inhibitor Screening Kit, β-Gal converts β-Gal substrate to give an intensely fluorescent product (Ex/Em = 480/520 nm). In the presence of a β-Gal inhibitor, the reaction is impeded/abolished resulting in decrease or total loss of fluorescence. This assay kit can be used to screen/study/characterize the potential inhibitors of Beta Galactosidase. The assay is simple, high-throughput adaptable and can be performed within 30 min.
• Data Sheet Link
• Additional description
  Tissue, pathway, proteinase, peptidase, protease ,acrosin, lipoprotein, activator, caspase, trypsin, papain, esterase inhibitors are proteins or receptor ligands or receptor antagonists that bind to an enzyme receptor and decreases its activity. Since blocking an enzyme's activity can kill a pathogen or correct a metabolic imbalance, many drugs are enzyme inhibitors. Not all receptor antagonist that bind to enzymes are inhibitors; enzyme activator ligands or agonists bind to enzymes and increase their enzymatic activity, while enzyme substrates bind and are converted to products in the normal catalytic cycle of the enzyme.

• Gene target
  Beta   Galactosidase   β-Gal   Inhibitor   Screening   Kit   Fluorometric  
• Short name
  Beta Galactosidase (β-Gal) Inhibitor Screening Kit (Fluorometric)
• Alternative name
  b Galactosidase (β-galanin/GMAP prepropeptide) suppressor detection reagent (fluorescence)
• Alternative technique
  kits
• Alternative to gene target
  v-kit Hardy-Zuckerman 4 feline sarcoma viral oncogene homolog, C-Kit and CD117 and PBT and SCFR, KIT and IDBG-18980 and ENSG00000157404 and 3815, transferase activity, Extracellular, Kit and IDBG-172083 and ENSMUSG00000005672 and 16590, KIT and IDBG-642326 and ENSBTAG00000002699 and 280832

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