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Category
Proteins
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Antibody Subtype
Recombinant Proteins
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Area of research
Signal Transduction
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Reactivity
Human
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Residues
18-517 amino acids: MSAAATQAVP APNQQPEVFC NQIFINNEWH DAVSRKTFPT VNPSTGEVIC QVAEGDKEDV DKAVKAARAA FQLGSPWRRM DASHRGRLLN RLADLIERDR TYLAALETLD NGKPYVISYL VDLDMVLKCL RYYAGWADKY HGKTIPIDGD FFSYTRHEPV GVCGQIIPWN FPLLMQAWKL GPALATGNVV VMKVAEQTPL TALYVANLIK EAGFPPGVVN IVPGFGPTAG AAIASHEDVD KVAFTGSTEI GRVIQVAAGS SNLKRVTLEL GGKSPNIIMS DADMDWAVEQ AHFALFFNQG QCCCAGSRTF VQEDIYDEFV ERSVARAKSR VVGNPFDSKT EQGPQVDETQ FKKILGYINT GKQEGAKLLC GGGIAADRGY FIQPTVFGDV QDGMTIAKEE IFGPVMQILK FKTIEEVVGR ANNSTYGLAA AVFTKDLDKA NYLSQALQAG TVWVNCYDVF GAQSPFGGYK MSGSGRELGE YGLQAYTEVK TVTVKVPQKN S
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Type of protein
Recombinant
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Expression System
Escherichia coli
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Grade Purity
Grater than 90% pure
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Concentration
1 mg/ml (lot specific)
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Form Buffer
Provided as a liquid in 20 mM Tris-HCl buffer, pH 7.5, containing 1 mM DTT, 1 mM EDTA, and 10% glycerol.
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Storage
Store at 4 deg C for short term storage (1/2 weeks). Aliquot and store at -20 deg C or - 70 deg C for long term storage. Avoid repeated freeze/thaw cycles.
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Shipping conditions
Blue Ice
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Tested for
SDS-PAGE
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Bioactivity
Specific activity is < 0.2 units/ml, and was obtained by measuring the increase of NADP in absorbance at 340 nm resulting from the reduction of NAD. One unit will oxidize 1.0 uM of acetaldehyde to acetic acid per minute at pH 8.0 at 25 DEG C in the presence of beta-NAD, potassium and thiols.
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Assay Information
1. Prepare a 3ml reaction mixture into a suitable container: The final concentrations are 103 mM Tris, 0.67 mM beta-NAD, 100 mM potassium chloride, 10 mM 2-mercaptoethanol, 2 mM acetaldehyde, 0.0007 % (w/v) BSA. 2. Equilibrate to 25 DEG C and monitor at A340nm until value is constant using a spectrophotometer. 3. Add 5ug of recombinant ALDH2 into reaction mixture and mix immediately. 4. Record the increase at A340nm for 5 minutes
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Additional Information
ALDH2 protein has been used in SDS PAGE and may be suitable for use in other assays to be determined by the end user.
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