STAT3 Antibody (phospho-S727)
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Catalog number
F44390-0.08ML
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Price
Please ask
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Size
0.08 ml
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Category
Antibody
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Concentration
In 1X PBS pH 7.4 with 0.09% sodium azide
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Form
Purified
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Conjugation
Unconjugated
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Clone
Polyclonal antibody
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Recognised antigen
STAT3 (phospho-S727)
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Host animal
Rabbit (Oryctolagus cuniculus)
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Clonality
Polyclonal (rabbit origin)
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Species reactivity
Human (Homo sapiens), Mouse (Mus musculus) ; Due to limited knowledge and inability to test the antibody against all known species, we cannot guarantee that no other cross reactivity can occur.
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Tested applications
IF, WB, ELISA
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Recommended dilutions
Immunofluorescence: 1:10-1:50,Western blot: 1:1000
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Notes
Titration of the phospho-STAT3 antibody may be required due to differences in protocols and secondary/substrate sensitivity.
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Intented use
This phospho-STAT3 antibodyis to be used only for research purposes and not for diagnostics..
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Uniprot
P40763
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Purity
Purified
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Description
STAT3 is a member of the STAT protein family. In response to cytokines and growth factors, STAT family members are phosphorylated by the receptor associated kinases, and then form homo- or heterodimers that translocate to the cell nucleus where they act as transcription activators. This protein is activated through phosphorylation in response to various cytokines and growth factors including IFNs, EGF, IL5, IL6, HGF, LIF and BMP2. This protein mediates the expression of a variety of genes in response to cell stimuli, and thus plays a key role in many cellular processes such as cell growth and apoptosis. The small GTPase Rac1 has been shown to bind and regulate the activity of this protein. PIAS3 protein is a specific inhibitor of this protein.
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Immunogen
This phospho-STAT3 antibody was produced from rabbits immunized with a KLH conjugated synthetic phosphopeptide corresponding to amino acid residues surrounding S727 of human STAT3.
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Storage
Aliquot the phospho-STAT3 antibody and store frozen at -20 deg. Celcius or lower. Cycles of freezing and thawing can denaturate the peptide chains of the antibodies and reduce their sensitivity and/or change their affinity. Prepare aliqotes in such a manner so that freeze-thaw cycles are minimized.
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Properties
If you buy Antibodies supplied by NJS poly they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.
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French translation
anticorps
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Gene target
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Gene symbol
STAT3
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Short name
Anti-STAT3 (phospho-S727)
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Technique
Antibody, antibodies against human proteins, antibodies for
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Isotype
Rabbit Ig
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Alternative name
Antibodies to STAT3 (phospho-S727)
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Alternative technique
antibodies
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Alternative to gene target
signal transducer and activator of transcription 3 (acute-phase response factor), ADMIO and APRF and HIES, STAT3 and IDBG-50702 and ENSG00000168610 and 6774, protein dimerization activity, nuclei, Stat3 and IDBG-211637 and ENSMUSG00000004040 and 20848, STAT3 and IDBG-640197 and ENSBTAG00000021523 and 101910056,508541
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Gene info
MeSH Data
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Name
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Concept
Scope note:
Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
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Tree numbers
- E05.196.401.143
- E05.301.300.096
- E05.478.566.320.200
- E05.601.262
- E05.601.470.320.200
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Qualifiers
ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data
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