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Synonyms
soluble IL-4 receptor alpha, CD124, IL-4R subunit alpha, IL-4R-alpha, IL-4RA, Soluble IL-4 receptor subunit alpha, Soluble IL-4R-alpha, sIL4Ralpha/prot, IL-4-binding protein, IL4-BP
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Alternative_names
soluble IL-4 receptor alpha, CD124, IL-4R subunit alpha, IL-4R-alpha, IL-4RA, Soluble IL-4 receptor subunit alpha, Soluble IL-4R-alpha, sIL4Ralpha/prot, IL-4-binding protein, IL4-BP
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Description
Plays an important role in regulating the differentiation of naive CD4 T cells and class switching to IgG1 and IgE.
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Recombinant
Yes
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Source
HEK293 cells
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Purity by SDS PAGE
≥ 98%
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Assay
SDS-PAGE
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Biological activity
The ED50 was determined by its ability to inhibit the IL-4 dependent proliferation of human TF-1 cells is ≤ 5.0 ng/ml (in the presence of 0.5 ng/ml of IL-4), corresponding to a specific activity of ≥ 2 x 10 AA Sequence
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Storage Temp
-20°C
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Shipping
Gel Pack
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Shelf Life
12 months
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Appearance
Lyophilized
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Reconstitution Instructions
Centrifuge the vial prior to opening. Reconstitute in water to a concentration of 0.1-1.0 mg/ml. Note: Slow to dissolve. Do not vortex. This solution can be stored at 2-8°C for up to 1 week. For extended storage, it is recommended to further dilute in a buffer containing a carrier protein (example 0.1% BSA) and store in working aliquots at -20°C to -80°C.
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Background Information
IL-4 can signal through type I and type II receptor complexes, which share a common γ chain (γc). The type I receptor contains in addition to the γ chain an IL-4Rα subunit, whereas the type II receptor contains the IL-13Rα. The secreted extracellular domain of IL-4Rα, called sIL-4Rα, binds IL-4 and antagonizes its activity. It plays an important role in regulating the differentiation of naive CD4 T cells and class switching to IgG1 and IgE. Recombinant human sIL-4Rα is a 209 amino acid protein which corresponds to the entire extracellular domain of IL-4Rα.
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Amino acid sequence
GNMKVLQEPT CVSDYMSIST CEWKMNGPTN CSTELRLLYQ LVFLLSEAHT CIPENNGGAG CVCHLLMDDV VSADNYTLDL WAGQQLLWKG SFKPSEHVKP RAPGNLTVHT NVSDTLLLTW SNPYPPDNYL YNHLTYAVNI WSENDPADFR IYNVTYLEPS LRIAASTLKS GISYRARVRA WAQCYNTTWS EWSPSTKWHN SYREPFEQH
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Handling
Centrifuge the vial prior to opening.
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Usage
For Research Use Only! Not to be used in humans
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Properties
Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.
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Additional source
Recombinants or rec. proteins
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Group
recombinants
