Recombinant Human GM-CSF/CSF2 (E. coli)
Recombinant Human Granulocyte-Macrophage Colony-Stimulating Factor is produced by our E.coli expression system and the target gene encoding Ala18-Glu144 is expressed.
Estimated molecular weight
Greater than 95% as determined by reducing SDS-PAGE.
Less than 0.1 ng/µg (1 IEU/µg) as determined by LAL test.
Lyophilized from a 0.2 µm filtered solution of 20mM PB, 150mM NaCl, 5% Mannitol, pH 7.2.
Lyophilized protein should be stored at below -20°C, though stable at room temperature for 3 weeks. Reconstituted protein solution can be stored at 4-7°C for 2-7 days. Aliquots of reconstituted samples are stable at below -20°C for 3 months.
See included datasheet or contact us for more information.
Granulocyte-macrophage colony-stimulating factor (GM-CSF), also known as colony stimulating factor 2 (CSF2), is a monomeric glycoprotein secreted by macrophages, T cells, mast cells, NK cells, endothelial cells and fibroblasts that functions as a cytokine. The pharmaceutical analogs of naturally occurring GM-CSF are called sargramostim and molgramostim. Reconstitution It is recommended that sterile ddH2O containing at least 0.1% human serum albumin or bovine serum albumin be added to the vial to prepare a stock solution of not less than 1 mg/mL of the cytokine.
Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.
Recombinants or rec. proteins
Recombinant GM-CSF/CSF2 ( )
Recombinant, E. coli recombinant proteins are genetic recombinations in Escherichia coli, supplied as white sterile powder lyopillized. novo advises they will be reconstituted in a buffer soluion or culture medium for cell culture.
e. coli, Escherichia coli, Recombination of bioactive proteins and longer peptides in Escherichia Coli is done often with His tagging. novo supplies Rec. E. Coli affinity purified or tag purified antigens in 1 quantities or bulk volumes on request.
E. coli, E. coli, Humans
recombinant H. sapiens GM-CSF/colony stimulating factor 2 (granulocyte-macrophage) (E. coli)
Alternative to gene target
colony stimulating factor 2 (granulocyte-macrophage), GMCSF, CSF2 and IDBG-41275 and ENSG00000164400 and 1437, this GO :0001892 and embryonic placenta development and biological process this GO :0005125 and cytokine activity and molecular function this GO :0005129 and granulocyte macrophage colony-stimulating factor receptor binding and molecular function this GO :0005515 and protein binding and molecular function this GO :0005576 and extracellular region and cellular component this GO :0005615 and extracellular space and cellular component this GO :0006955 and immune response and biological process this GO :0008083 and growth factor activity and molecular function this GO :0008284 and positive regulation of cell proliferation and biological process this GO :0010468 and regulation of gene expression and biological process this GO :0010628 and positive regulation of gene expression and biological process this GO :0010744 and positive regulation of macrophage derived foam cell differentiation and biological process this GO :0032747 and positive regulation of interleukin-23 production and biological process this GO :0042045 and epithelial fluid transport and biological process this GO :0042116 and macrophage activation and biological process this GO :0042127 and regulation of cell proliferation and biological process this GO :0042523 and positive regulation of tyrosine phosphorylation of Stat5 protein and biological process this GO :0043011 and myeloid dendritic cell differentiation and biological process this GO :0045740 and positive regulation of DNA replication and biological process this GO :0045918 and negative regulation of cytolysis and biological process this GO :0071222 and cellular response to lipopolysaccharide and biological process this GO :0071803 and positive regulation of podosome assembly and biological process this GO :0097028 and dendritic cell differentiation and biological process this GO :2001240 and negative regulation of extrinsic apoptotic signaling pathway in absence of ligand and biological process, growth factor activity, this GO :0005125: cytokine activity and also this GO :0005129: granulocyte macrophage colony-stimulating factor receptor binding and also this GO :0005515: protein binding and also this GO :0008083: growth factor activity, this GO :0005125: cytokine activity, this GO :0005129: granulocyte macrophage colony-stimulating factor receptor binding, this GO :0005515: protein binding, this GO :0008083: growth factor activity, Extracellular, Csf2 and IDBG-172875 and ENSMUSG00000018916 and 12981, CSF2 and IDBG-644701 and ENSBTAG00000001570 and 281095
1. Gene info
Scope note:The initial culturing of cells derived directly from fresh TISSUES.
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