Anti-5-Hydroxymethylcytosine Antibody
-
Catalog number
A1295-50
-
Price
Please ask
-
Size
50 µg
-
-
Antibody Target
5-hmc
-
Target Alternative Name
5-hmc, 5hmc
-
Antibody Type
Monoclonal
-
Clone
4D9
-
Species Reactivities
Human, Mouse, Rat
-
Immunogen Sequence
5-hydroxymethylcytosine (5-hmC) is a modified base form of cytosine recently found in human/mouse brain and in embryonic stem cells. This DNA pyrimidine nitrogen base can be generated by oxidation of 5-methylcytosine, a reaction mediated by the ten-eleven translocation (TET) family of the 5-mC hydroxylases. The function of this base is still not elucidated but it is believed to play an important role in switching genes on and off.
-
Appearance
Colorless liquid
-
Form
Liquid
-
Formulation
Phosphate Buffer 10mM - NaCl 0.15 M - pH 7.5
-
Purification
Affinity purified
-
Application
ELISA, hMeDIP IF, Dot Blot
-
Positive Control
Dot Blot, ELISA
-
Application And Usages
ELISA: 1:1000, hMeDIP: 1-3 µg per IP, IF: 1:500, Dot Blotting: 1:2,000
-
Description
5-hydroxymethylcytosine (5-hmC) is a modified base form of cytosine recently found in human/mouse brain and in embryonic stem cells. This DNA pyrimidine nitrogen base can be generated by oxidation of 5-methylcytosine, a reaction mediated by the ten-eleven translocation (TET) family of the 5-mC hydroxylases. The function of this base is still not elucidated but it is believed to play an important role in switching genes on and off.
-
Storage Condition
-20 °C
-
Shipping Condition
gel pack
-
Shelf Life
12 months
-
Usage
For Research Use Only! Not to be used in humans
-
Handling
The antibody solution should be gently mixed before use
-
Western Blot Verified
Verified
-
Immunocytochemistry Verified
Not Verified
-
Immunofluorescence Verified
Not Verified
-
Immunoprecipitation Verified
Not Verified
-
FACS Verified
Not Verified
-
ELISA Verified
Not Verified
-
ChIP Verified
Not Verified
-
Dot Blot Verified
Verified
-
Flow Cytometry Verified
Not Verified
-
-
Additional description
This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided. Antibody for research use.
-
Properties
If you buy Antibodies supplied by Biovision they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.
-
French translation
anticorps
-
Gene target
-
Gene symbol
HMCES
-
Short name
Anti-5-Hydroxymethylcytosine Antibody
-
Technique
Antibody, anti-, anti, antibody to, antibodies, antibodies against human proteins, antibodies for
-
Isotype
Mouse IgG1
-
Alternative name
Antibody to5-Hydroxymethylcytosine (antibody to-)
-
Alternative technique
antibodies
-
Gene info
MeSH Data
-
Name
-
Concept
Scope note:
Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
-
Tree numbers
- E05.196.401.143
- E05.301.300.096
- E05.478.566.320.200
- E05.601.262
- E05.601.470.320.200
-
Qualifiers
ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data
Product images
Similar products