Rabbit anti Sheep albumin
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Catalog numberRASh/Alb
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Price:
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Size1 mL
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CategoryPrimary Antibodies
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Long descriptionThe defined antibody specificity is directed to albumin as tested against sheep sera. In immunoelectrophoresis and double radial immunodiffusion (Ouchterlony), using various antiserum concentrations against appropriate concentrations of the immunogen, a single characteristic precipitin line is obtained which shows a reaction of identity with the precipitin lines obtained against sheep serum and the purified albumin. As precipitating antiserum to identify or measure sheep albumin by a variety of immunodiffusion techniques, including immunoelectrophoresis, single and double radial immunodiffusion (Mancini, Ouchterlony) and electroimmunodiffusion (Laurell). It has not been tested for use in nephelometry, ELISA or immunochemistry, but this does not exclude such use if proper controls are included.
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Antibody come fromHighly purified albumin isolated from sheep serum. Freund’s complete adjuvant is used in the first step of the immunization procedure.
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Other descriptionDelipidated, heat inactivated, lyophilized, stable whole antiserum. No preservative added. Total protein and IgG concentrations in the antiserum are comparable to those in pooled rabbit serum. No foreign proteins added.
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ClonePolyclonal
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Antigen antibody binding interactionRabbit anti Sheep albumin Antibody
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Antibody is raised inRabbit
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Antibody s reacts withSheep
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Antibody s reacts with these speciesInter-species cross-reactivity is a normal feature of antibodies to Serum proteins, since homologous proteins of different species frequently share antigenic determinants. The degree of cross-reactivity is also dependent on the concentRations of the reactants and the sensitivity of the assay arrangement. This antiSerum has been tested in double radial immunodiffusion with the following results: Bovine ++ Goat +++ Human + Rabbit - Chicken - Guinea Pig + Monkey + Rat + Dog ++ Horse + Mouse ± Swine ++
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Antibody s specificityPrecipitating polyclonal Rabbit antiSerum to Sheep Serum albumin.
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Research interestVeterinary
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ApplicationImmunoprecipitation
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Antibody s suited forImmunoprecipitation. In immunoelectrophoresis use 2 μl serum, plasma or equivalent against 120 μl antiserum. In double radial immunodiffusion (Ouchterlony) use a rosette arrangement with 10 μl antiserum in a 3 mm diameter centre well and 2 μl serum samples (neat and serially diluted) in 2 mm diameter peripheral wells. In single radial immunodiffusion and electroimmunodiffusion use 0.5 to 1.0 percent antiserum in the agar gel. Precipitin titre not less than 1:64 when tested against normal sheep serum in agar block immunodiffusion titration.
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StorageThe lyophilized antiserum is shipped at ambient temperature and may be stored at +4°C; prolonged storage at or below -20°C. Reconstitute the lyophilized antiserum by adding 1 ml sterile distilled water. Dilutions may be prepared by adding phosphate buffered saline (PBS, pH 7.2). Repeated thawing and freezing should be avoided. If a slight precipitation occurs upon storage, this should be removed by centrifugation. It will not affect the performance of the antiserum. Diluted antiserum should be stored at +4°C, not refrozen, and preferably used the same day. Lyophilized at +4°C--at least 10 years. Reconstituted at or below -20°C--3-5 years. Reconstituted at +4°C--7 days
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Relevant referencesno information yet
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Protein numbersee ncbi
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WarningsThis product is intended FOR RESEARCH USE ONLY, and FOR TESTS IN VITRO, not for use in diagnostic or therapeutic procedures involving humans or animals. This product contains sodium azide. To prevent formation of toxic vapors, do not mix with strong acidic solutions. To prevent formation of potentially explosive metallic azides in metal plumbing, always wash into drain with copious quantities of water. This datasheet is as accurate as reasonably achievable, but Nordic-MUbio accepts no liability for any inaccuracies or omissions in this information.
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DescriptionThis antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided.
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AboutRabbits are used for polyclonal antibody production by nordc. Rabbit antibodies are very stable and can be stored for several days at room temperature. nordc adds sodium azide and glycerol to enhance the stability of the rabbit polyclonal antibodies. Anti-human, anti mouse antibodies to highly immunogenic selected peptide sequences are" monoclonal like" since the epitope to which they are directed is less than 35 amino acids long.
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Latin nameOryctolagus cuniculus
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Gene target
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Gene symbolALB, DBP, HNF1A
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Short nameRabbit anti albumin
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TechniqueRabbit, anti, antibody to
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HostRabbit, Rabbits
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Isotypenot specified
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Labelunlabeled
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SpeciesSheep, The sheep (Ovis aries) ELISAs are detecting sheep proteins and antigens. Some epitopes will also recognize goat proteins. Sheep and goats are also used to produce polyclonals. An adult female sheep is referred to as a ewe a male as a ram, a castrated male as a wether, and a younger sheep as a lamb. Sheeps
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Alternative nameproduction species: rabbit antibody to ovine albumin
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Alternative techniquerabbit-anti, antibodies
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Gene info
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Identity
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Gene
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Long gene namealbumin
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GenBank acession
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Locus
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Discovery year1986-01-01
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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VEGA ID
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Locus Specific Databases
Gene info
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Identity
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Gene
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Long gene nameD-box binding PAR bZIP transcription factor
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Synonyms gene name
- D site of albumin promoter (albumin D-box) binding protein
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Synonyms
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GenBank acession
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Locus
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Discovery year1991-11-27
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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Classification
- PAR bZIP family
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VEGA ID
Gene info
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Identity
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Gene
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Long gene nameHNF1 homeobox A
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Synonyms gene
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Synonyms gene name
- transcription factor 1, hepatic; LF-B1, hepatic nuclear factor (HNF1), albumin proximal factor
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Synonyms
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GenBank acession
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Locus
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Discovery year1990-02-12
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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Classification
- HNF class homeoboxes
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VEGA ID
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Locus Specific Databases
MeSH Data
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Name
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ConceptScope note: Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
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Tree numbers
- E05.196.401.143
- E05.301.300.096
- E05.478.566.320.200
- E05.601.262
- E05.601.470.320.200
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Qualifiersethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data