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Availability
Please contact us to check the availability
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Immunogen
1. Human recombinant AMACR (P504S) protein, whole sequence (for rabbit monoclonal antibody 13H4); 2. Recombinant human p63 protein aa 1-205 (for mouse monoclonal antibody 4A4); 3. Cytokeratin extract of human stratum corneum (for mouse monoclonal antibody 34ßE12)
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Specificity
1. Human AMACR (P504S) protein, Alpha-Methylacyl CoA Racemase, 2. human p63 nuclear protein, and 3. high molecular weight cytokeratins (56-57 and 66-68 kDa)
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Species reactivity
Human
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Product presentation
Affinity purified antibodies in PBS, BSA, sodium azide (0.09%)**. Use antibody dilution buffer (e.g. Art. No. PU002) containing sufficient protein and preservative.
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Product category
Primary Antibodies
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Antibody s raised in
Rabbit/Mouse
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Antibody s isotype
IgG / IgG2a / IgG1
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Antibody s clone
13H4 / 4A4 / 34ßE12
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Product is supplied as
ready to use
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Recommended concentration for use
neat
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Protein concentration
See the included datasheet
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Positive control
Prostatic intraepithelial neoplasia
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Pretreatment
Use formalin-fixed and paraffin-embedded sections; Retrieval conditions: Unmasking fluid T, TEC buffer (Tris/EDTA/Citrate) pH 8 (Art. No. DE005) in a pressure cooker at 100°C 20-40 minutes
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Tested applications
IHC(C, P)
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Recommended secondary reagent
Detection methods: VECTASTAIN Universal Elite ABC or ImmPRESS Mouse IgG/ Rabbit IgG or EnVision Plus kits detecting rabbit and mouse IgG simultaneously.
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Storage method
Store at 2-8°C
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UniProt number
Go to UniProt/SwissProt
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Litterature
1. Jiang Z, Woda BA, Rock KL et al. (2001) P504S: a new molecular marker for the detection of prostate carcinoma. Am J Surg Pathol 25:1397-1404. 2. Weinstein MH, Signoretti S, Loda M (2002) Diagnostic utility of immunohistochemical staining for p63, a sensitive marker of prostatic basal cells. Mod Pathol. Dec;15(12):1302-8. 3. Luo J, Shan Zha, Wesley R, et al. (2002) Alpha methylacyl-CoA recemase, a new molecular marker for prostate cancer. Cancer Res 62:2220-2226. 4. Yang X.J., Lecksell K., Gaudin P., and Epstein J.I. (1999) Rare expression of high-molekular-weight cytokeratin in adenocarcinoma of the prostate gland: a study of 100 cases of metastatic and locally advanced prostate cancer. Am. J. Surg. Pathol. 23(2); 147-152.
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Tips
*Gentaur's antibodies are intended for in vitro research use only. They must not be used for clinical diagnostics and not for in vivo experiments in humans or animals. ** The preservative sodium azide is known to be poisonous and potentially hazardous to health. It should be handled only by trained staff. Despite of the product's low azide concentration it must be handled with care. Dispose according to regional rules!
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About
Immunoglobulin gamma, IgG, mouse monoclonal H&L chain clones or rabbit, goat polyclonal antibodies have 4 parts. There are 2 heavy chains, 2 light chains. The IgG antibody has 2 antigen binding sites. They represent 70% or more of serum antibodies. This antibody can be antigen purified or protein A or G purified. For storage sodium azide is added or you can call us to request azide free antibody preparations. These will need colder storage temperatures. Monoclonals of this antigen are available in different clones. Each murine monoclonal anibody has his own affinity specific for the clone. Mouse monoclonal antibodies are purified protein A or G and can be conjugated to FITC for flow cytometry or FACS and can be of different isotypes. Rabbits are used for polyclonal antibody production by bio logo. Rabbit antibodies are very stable and can be stored for several days at room temperature. bio logo adds sodium azide and glycerol to enhance the stability of the rabbit polyclonal antibodies. Anti-human, anti mouse antibodies to highly immunogenic selected peptide sequences are" monoclonal like" since the epitope to which they are directed is less than 35 amino acids long.
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Test
Mouse or mice from the Mus musculus species are used for production of mouse monoclonal antibodies or mabs and as research model for humans in your lab. Mouse are mature after 40 days for females and 55 days for males. The female mice are pregnant only 20 days and can give birth to 10 litters of 6-8 mice a year. Transgenic, knock-out, congenic and inbread strains are known for C57BL/6, A/J, BALB/c, SCID while the CD-1 is outbred as strain.
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Latin name
Mus musculus, Oryctolagus cuniculus