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Category
Primary Antibodies
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Long description
Sirtuin 1 (Sirt 1) is encoded by a gene that encodes a number of the sirtuin-like proteins, all homologs to the yeast Sir2 protein. Members of the sirtuin family are characterized by a sirtuin core domain. Yeast sirtuin proteins are known to regulate epigenetic gene silencing, suppress recombination of DNA and repressess rDNA transcription by the RNA pol. Studies suggest that the human sirtuins regulate apoptosis by impairing proapoptotic ability and modulating cell senescence. They regulate muscle differentiation by deacetylating key proteins. They also deacetylate the following: 'Lys-382' of p53/TP53, TAF1B, 'Lys-266' of SUV39H1 (leading to its activation), 'Lys-26' of HIST1H1E, APEX1 at 'Lys-6' and 'Lys-7' and 'Lys-16' of histone H4 (in vitro), as well as H2A. Sirtuins are also involved in HES1- and HEY2-mediated transcriptional repression and inhibit skeletal muscle differentiation by deacetylating PCAF and MYOD1. They may serve as a sensor of the cytosolic ratio of NAD+/NADH, which is essential in skeletal muscle cell differentiation. The eNoSC complex is able to sense the energy status of cell: upon glucose starvation, elevation of NAD+/NADP+ ratio activates SIRT1, leading to histone H3 deacetylation followed by dimethylation of H3 at 'Lys-9' (H3K9me2) by SUV39H1 and the formation of silent chromatin in the rDNA locus. In the case of HIV-1 infection, sirtuin interacts with and deacetylates the viral Tat protein. Cellular AP endonuclease activity is stimulated by promoting the association of APEX1 to XRCC1. Red wine, which contains resveratrol, may participate in activation of sirtuin proteins, and may therefore participate in an extended lifespan as observed in yeast.
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Antibody come from
Synthetic peptide derived from human neurogenin-1 protein.
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Other description
Provided as solution in phosphate buffered saline with 0.08% sodium azide. Antigen Immunoaffinity Purification
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Clone
Polyclonal
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Antigen antibody binding interaction
Rabbit anti Sirt1 Protein (Human) Antibody
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Antibody is raised in
Rabbit
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Antibody s reacts with
Human
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Antibody s reacts with these species
This antibody doesn't cross react with other species
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Antibody s specificity
No Data Available
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Application
Immunohistochemistry, Western Blot, ELISA
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Antibody s suited for
Antibody can be used for immunohistochemical staining (1-5 µg/ml). Optimal concentration should be evaluated by serial dilutions.
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Storage
-20ºC
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Relevant references
1. Velkey, J.M., et al. ÔExpression of Neurogenin 1 in Mouse Embryonic Stem Cells Directs the Differentiation of Neuronal Precursors and Identifies Unique Patterns of Down-stream Gene Expression.Õ Dev. Dyn., 242, 230-253 (2013)_x000B__x000B_2. Hill, G.W., et al. ÔNetrin-1-mediated axon guidance in mouse embryonic stem cells overexpressing neurogenin-1.Õ Stem Cells Dev., 21, 2827-2837 (2012)_x000B__x000B_3. Choi, C.I., et al. ÔNeural Induction with Neurogenin 1 Enhances the Therapeutic Potential of Mesenchymal Stem Cells in an ALS Mouse Model.Õ Cell Transplant., Epub ahead of print (2012)
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Protein number
see ncbi
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Warnings
This product is intended FOR RESEARCH USE ONLY, and FOR TESTS IN VITRO, not for use in diagnostic or therapeutic procedures involving humans or animals. This datasheet is as accurate as reasonably achievable, but Nordic-MUbio accepts no liability for any inaccuracies or omissions in this information.
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Description
This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided.
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Properties
Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.
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About
Rabbits are used for polyclonal antibody production by nordc. Rabbit antibodies are very stable and can be stored for several days at room temperature. nordc adds sodium azide and glycerol to enhance the stability of the rabbit polyclonal antibodies. Anti-human, anti mouse antibodies to highly immunogenic selected peptide sequences are" monoclonal like" since the epitope to which they are directed is less than 35 amino acids long.
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Latin name
Oryctolagus cuniculus
