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Category
Primary Antibodies
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Long description
Amyloid (A4) Precursor Protein (APP) is a ubiquitously expressed transmembrane protein that is sequentially cleaved by -secretase and -secretase to release extracellular peptides such as the -amyloid peptides, which are deposited in the brain in Alzheimer's disease. APP exists in several isoforms ranging from 100-140 kDa, and is phosphorylated on various residues within the extracellular and cytoplasmic domains, effecting the proteolytic processing and secretion of the protein. Threonine 668 can be phosphorylated by cyclin-dependent kinase 5 (cdk5), GSK-3 and JNK, and may play an important role in neurite outgrowth of differentiating neurons.
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Antibody come from
Synthetic phosphopeptide derived from the human VEGFR2 protein which contains Y951.
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Other description
Provided as solution in phosphate buffered saline, pH 7.3, with 1.0 mg/ml BSA and 0.05% sodium azide. Purified from rabbit serum by sequential epitope-specific chromatography. The antibody has been negatively preadsorbed using a non-phosphopeptide corresponding to the site of phosphorylation to remove antibody that is reactive with non-phosphorylated APP. The final product is generated by affinity chromatography using an APP-derived peptide that is phosphorylated at threonine 668.
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Clone
Polyclonal
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Antigen antibody binding interaction
Rabbit anti phospho Amyloid Precursor Protein [pT668] Antibody
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Antibody is raised in
Rabbit
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Antibody s reacts with
Human
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Antibody s reacts with these species
This antibody doesn't cross react with other species
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Antibody s specificity
No Data Available
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Application
Western Blot
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Antibody s suited for
Antibody can be used for Western blotting (1:1000 dilution). For Western blotting applications, we recommend using the antibody at 0.25-1.0 µg/mL. At 0.40 µg/mL, the dilution provides 100 mL working solution, which at 10 mL/blot allows 10 blots to be performed. The optimal antibody concentration should be determined for each specific application.
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Storage
-20ºC
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Relevant references
1. Zeng, H., et al. (2001) Tyrosine residues 951 and 1059 of vascular endothelial growth factor receptor-2 (KDR) are essential for vascular permeability factor/vascular endothelial growth factorinduced endothelium migration and proliferation, respectively. J. Biol. Chem._x000B_276(35):32714-32719._x000B__x000B_2. Rahimi, N., et al. (2000) Receptor chimeras indicate that the vascular endothelial growth factor receptor-1 (VEGFR-1) modulates mitogenic activity of VEGFR-2 in endothelial cells. J. Biol. Chem. 275(22):16986-16992. _x000B__x000B_3. Shalaby, F., et al. (1995) Failure of blood-island formation and vasculogenesis in Flk-1-deficient mice. Nature 376(6535):62-66.
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Protein number
see ncbi
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Warnings
This product is intended FOR RESEARCH USE ONLY, and FOR TESTS IN VITRO, not for use in diagnostic or therapeutic procedures involving humans or animals. This datasheet is as accurate as reasonably achievable, but Nordic-MUbio accepts no liability for any inaccuracies or omissions in this information.
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Description
This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided. Precursor cell, also called a blast cell or simply blast, is a type of partially differentiated, usually unipotent cell that has lost most or all of the stem cell multipotency.
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About
Rabbits are used for polyclonal antibody production by nordc. Rabbit antibodies are very stable and can be stored for several days at room temperature. nordc adds sodium azide and glycerol to enhance the stability of the rabbit polyclonal antibodies. Anti-human, anti mouse antibodies to highly immunogenic selected peptide sequences are" monoclonal like" since the epitope to which they are directed is less than 35 amino acids long.
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Latin name
Oryctolagus cuniculus
