-
Category
Primary Antibodies
-
Long description
Janus Activating Kinase 2 (JAK2) is a 130 kDa tyrosine kinase involved in cytoplasmic signal transduction. Ligand binding to a variety of cell surface receptors (e.g., cytokine, growth factor, GPCRs) leads to an association of those receptors with JAK proteins, which are then activated via phosphorylation on tyrosines 1007 and 1008 in the kinase activation loop. Activated JAK proteins phosphorylate and activate STAT (signal transducers and activators of transcription) proteins, which then dimerize and translocate to the nucleus. Once in the nucleus, STAT proteins bind to DNA and modify the transcription of various genes, which can lead to various responses such as cell proliferation, cell survival and differentiation. The JAK2 antibody is useful as a positive control and for measuring total JAK2 protein levels to compare signals obtained with the JAK2 PSSA.
-
Antibody come from
The antibody was produced using a synthetic peptide from the internal region of the human PC-specific PL-D1 protein.
-
Other description
Provided as solution in phosphate buffered saline, pH 7.2, with 1.0 mg/ml BSA and 0.05% sodium azide. Protein A/G Chromatography
-
Clone
Polyclonal
-
Antigen antibody binding interaction
Rabbit anti JAK2 Antibody
-
Antibody is raised in
Rabbit
-
Antibody s reacts with
Human, Mouse, Rat
-
Antibody s reacts with these species
This antibody doesn't cross react with other species
-
Antibody s specificity
No Data Available
-
Application
Western Blot
-
Antibody s suited for
This antibody has been used in Western blot applications. For Western blot applications, we recommend using the antibody at a dilution of 1:100 to 1:2000 against purified recombinant protein. Due to the low expression levels of this protein, it is suggested that the protein be immunoprecipitated before Western blotting. The optimal antibody concentration should be determined for each specific application.
-
Storage
-20ºC
-
Relevant references
1. Hammond S.M., et al. (1997) Characterization of two alternately spliced forms of phospholipase D1. Activation of the purified enzymes by phosphatidylinositol 4,5-bisphosphate, ADP-ribosylation factor, and Rho family monomeric GTP-binding proteins and protein kinase C-alpha. J. Biol. Chem. 272 (6):3860-3868._x000B__x000B_2. Sciorra, V.A. and A.J. Morris (1999) Sequential actions of phospholipase D and phosphatidic acid phosphohydrolase 2b generate diglyceride in mammalian cells. Mol. Biol. Cell 10:3863-3876 (cites the use of the PLD1 and PD2 antibodies).
-
Protein number
see ncbi
-
Warnings
This product is intended FOR RESEARCH USE ONLY, and FOR TESTS IN VITRO, not for use in diagnostic or therapeutic procedures involving humans or animals. This datasheet is as accurate as reasonably achievable, but Nordic-MUbio accepts no liability for any inaccuracies or omissions in this information.
-
-
Description
This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided.
-
About
Rabbits are used for polyclonal antibody production by nordc. Rabbit antibodies are very stable and can be stored for several days at room temperature. nordc adds sodium azide and glycerol to enhance the stability of the rabbit polyclonal antibodies. Anti-human, anti mouse antibodies to highly immunogenic selected peptide sequences are" monoclonal like" since the epitope to which they are directed is less than 35 amino acids long.
-
Latin name
Oryctolagus cuniculus
