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ELISA s application
For quantitative measurement of mouse Leptin in cell culture supernatant, cell lysate, tissue homogenates, serum and plasma heparin, EDTA .
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Gene tested
murine Leptin
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ELISA standard curve
Concentration(pg/ml) + 0 + 62.5 + 125 + 250 + 500 + 1000 + 2000 + 4000 O.D. + 0.104 + 0.172 + 0.213 + 0.241 + 0.411 + 0.923 + 1.135 + 1.714
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ELISA kit detection limits
62.5pg/ml to 4000pg/ml
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ELISA s sensitivity
< 10pg/ml
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Target of this ELISA
Natural and recombinant mouse Leptin
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Cross reaction
the ELISA does not cross react with any other proteins except from closely related species
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ELISA s precision parameters
intra assay precisions and inter assay precisions samples;1;2;3;1;2;3 n;16;16;16;24;24;24 mean (pg/ml);196;1123;2253;216;1339;2487 ELISA standard deviations 8.82;58.4;135.2;12.1;83.02;174.1 CV(%);4.5;5.2;6;5.6;6.2;7
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ELISA kit description
GENTAUR's mouse Leptin ELISA test is a ready to use ELISA kit. We use a monoclonal from rat specific for Leptin was used as capure antibodies on the ELISA test plate. The internal standards (E.coli, V22-C167) and your samples are measured with a biotin conjugated polyclonal from goat specific for Leptin will than be washed with PBS. The colour reaction with TMB on HRP will colour the well from blue to yellow with the stop solution. The more mouse Leptin in your sample the higher the ELISA signal will be compared to this elisa's standard curve.
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Detector and capure antibodies
A rat monoclonal detector antibodies is used with a goat biotin polyconal capture antibodies.
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The ELISA contains
Catalog number;Description;Quantity -;96-well plate precoated with anti- mouse Leptin antibodies;1 ST0000-10;Lyophilized recombinant mouse Leptin standard;10ng/tubex2 AR1107;Biotinylated anti- mouse Leptin antibodies;130ul(dilutions 1:100) AR1103;Avidin-Biotin-Peroxidase Complex(ABC);130ul(dilutions 1:100) AR1106-1;Sample diluent buffers;30ml AR1106-2;antibodies diluent buffers;12ml AR1106-3;ABC diluent buffers;12ml AR1104;TMB color developing agents;10ml AR1105;TMB stop solution;10ml -;Adhesive cover;4
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Anti coagulation
heparin or EDTA
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How the elisa works
Sample the amount of 100ul/well of the standard solution at 4000pg/ml, 2000pg/ml, 1000pg/ml, 500pg/ml, 250pg/ml, 125pg/ml, 62.5pg/ml mouse Leptin standard solutions into the precoated 96-well plate. Add 0.1ml of the sample diluent buffers into the control well (Zero well). Add 0.1ml of each properly diluted sample of mouse cell culture supernatant, serum or plasma(heparin, EDTA) to the ELISA plate. Keep in mind that each mouse Leptin standard solution and each sample be measured in duplicate.
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Minutes of TMB incubation
15-20
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More gene info
Leptin(or obese, OB) is a circulating hormone that is expressed abundantly and specifically in the adipose tissue. Mouse ob protein is a 166-amino acid polypeptide with a putative signal sequence and is 84 and 83% homologous to the mouse and rat ob proteins, respectively. Northern blot analysis reveals that OB RNA is present at a high level in adipose tissue but at much lower levels in placenta and heart. Indeed, Leptin is first described as an adipocyte-derived signalling factor, which, after interaction with its receptors, induces a complex response including control of bodyweight and energy expenditure. Leptin seems in addition to its role in metabolic control to have important roles in reproduction and neuroendocrine signalling. Leptin is a powerful inhibitor of bone formation in vivo. Comparative mapping of mouse and mouse DNA indicates that the ob gene is located within a region of mouse chromosome 6 that is homologous to a portion of mouse chromosome 7q. The standard product used in this kit is recombinant mouse Leptin.
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Antigen
E.coli, V22-C167
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Keep at
Store at 4˚C for 6 months, at -20˚C for 12 months. Avoid multiple freeze-thaw cycles(Shipped with wet ice.)
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Uniprot
Leptin
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Gene name
leptin
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Alternative
FLJ94114;_x000D_LEP;_x000D_LEP_HUMAN;_x000D__x000D__x000D_Leptin;_x000D_Leptin Murine Obesity Homolog;_x000D_Leptin Precursor Obesity Factor;_x000D_OB;_x000D_Obese protein;_x000D_Obesity factor;_x000D_Obesity homolog mouse ;_x000D_Obesity murine homolog leptin;_x000D_OBS
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Gen protein identity
P41160
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Entrez nr
16846
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Properties
E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays, E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays
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Test
ELISA Enzyme-linked immunosorbent assays Code 90320007 SNOMED, Mouse or mice from the Mus musculus species are used for production of mouse monoclonal antibodies or mabs and as research model for humans in your lab. Mouse are mature after 40 days for females and 55 days for males. The female mice are pregnant only 20 days and can give birth to 10 litters of 6-8 mice a year. Transgenic, knock-out, congenic and inbread strains are known for C57BL/6, A/J, BALB/c, SCID while the CD-1 is outbred as strain.
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Gene
Human or mouse Leptin (from Greek λεπτός leptos, "thin") the "satiety hormone", is a hormone made by adipose cells that helps to regulate energy balance by inhibiting hunger. Leptin is opposed by the actions of the hormone ghrelin, the "hunger hormone". Both hormones act on receptors in the arcuate nucleus of the hypothalamus to regulate appetite to achieve energy homeostasis. ELISA kits and peptides and antibodies are available.
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Latin name
Mus musculus
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Gene target
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Gene symbol
LEP
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Short name
Mouse Leptin PicoKine ELISA Kit
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Technique
ELISA kit, ELISA, Mouse, ELISAs Enzyme-linked immunosorbent assay 90320007 SNOMED code are used by the medical researcher for detection of human, mouse, … proteins are supplied in coated 96 well plates to be stored at +4°C. ELISA test kits can be sandwich ELISA. In sandwich ELISAS Enzyme-linked immunosorbent assay 9we use captor and detector antibodies. In single antibody ELISAs the antigen is coated and only a detector antibody is used. Traditional competition antigen ELISAs are coated with a captor antibody and a competitive antigen is labelled with the chromogen. In this case the ratio on your graph will be inverse proportional with the antigen present in your sample. A sign detected by the Colorimetric detection OD optical density absorbance at 450 nm between 0 and 4 with an ELISA reader for 450 nm absortion. Genprice Inc. the recognized authority tax ID 45-4304622 D-U-N-S number - 078440800. MeSH code E05 478 566 350 170 Enzyme-Linked Immunosorbent Assay. PROMT code 3841327 Immunoabsorbent assay tests. ELISA tests are enzyme linked immunoassays to detect human, mouse or other proteins in serum, plasma, urine or biological fluids. MeSH code E05 478 566 350 170 for Enzyme-Linked Immunosorbent Assay or ELISA kits. Also Enzyme-linked immunoassay, competitive have the SNOMED name 61461007 and the PROMT code for ELISA readers is 3841330. This is the desigation of other diagnostic analyzers and tests. mouses
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Host
mouse, antigen assay ELISA kit 1 Kit (96 Wells)
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Species
Mouse, Mouses
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Alternative name
Mouse Leptin PicoKine Enzyme-linked immunosorbent assay reagent
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Alternative technique
murine, elisas, kits
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Alternative to gene target
v-kit Hardy-Zuckerman 4 feline sarcoma viral oncogene homolog, C-Kit and CD117 and PBT and SCFR, KIT and IDBG-18980 and ENSG00000157404 and 3815, transferase activity, Extracellular, Kit and IDBG-172083 and ENSMUSG00000005672 and 16590, KIT and IDBG-642326 and ENSBTAG00000002699 and 280832
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