Hydroxyproline Assay Kit (Perchlorate-Free)

• Catalog number
  K226-100
• Price
  Please ask
• Size
  100 Reactions

• Description
  This kit does not use PCA to detect and quantify Hydroxyproline
• Summary
  • Detection method- Absorbance (560 nm) • Species reactivity- Mammalian • Applications- The assay is useful over the range of 0.1-1 μg.
• Detection Method
  Absorbance (OD = 560 nm).
• Species Reactivity
  Mammalian
• Applications
  Estimation of hydroxyproline concentration in various biological samples
• Sample Type
  • Animal tissues • protein/peptide hydrolysates • serum • urine
• Features Benefits
  • Simple procedure; takes less than ~60 minutes • Convenient and easy to use • Faster than Perchlorate-Based Assay • PCA-free
• Storage Conditions
  -20°C
• Shipping Conditions
  gel pack
• Shelf life
  12 months
• Background
  Hydroxyproline ((2S,4R)-4-hydroxyproline, Hyp) is a nonproteinogenic amino acid formed by the enzyme prolyl-4-hydroxylase, which catalizes the post-translational modification of polypeptide proline residues following protein synthesis. In animals, hydroxyproline is found almost entirely in collagen, which contains approximately 12-14% hydroxyproline by mass (the fibrilous protein elastin also contains small quantities of hydroxyproline). Hydroxyproline in tissue hydrolysates is thus a direct measure of the amount of collagen present. Collagen is the most abundant protein in mammals and many diseases are believed to affect collagen turnover, including tumor invasion and metastasis, rheumatoid arthritis, cardiopulmonary fibrosis and muscular dystrophy. The classical assay for determination of Hyp is based upon oxidation of Hyp to a pyrrole intermediate, followed by reaction with Ehrlich’s reagent dissolved in concentrated perchloric acid (HClO4). Perchloric acid is a hazardous material that is both toxic and highly reactive, requiring special handling and waste-disposal protocols. BioVision’s Hydroxyproline Assay Kit II (Perchlorate-Free) uses a proprietary acidic developer solution to accurately measure Hyp in tissue and protein/peptidase hydrolysates without the use of hazardous perchlorates. It is a quick and convenient protocol, yielding a brightly-colored chromophore with an absorbance peak at 560 nm. The assay can detect a minimum of 0.05 µg hydroxyproline per well in a 96-well format and generates colorimetric results that are virtually identical to the classical perchloric acid-based method.
• Data Sheet Link
• Test
  Biovision supplies other types of Assays as 1.

• Gene target
  Hydroxyproline   Kit   Perchlorate-Free  
• Short name
  Hydroxyproline Assay Kit (Perchlorate-Free)
• Technique
  Assay, assays
• Host
  Assay
• Alternative name
  Hydroxyproline test reagent (Perchlorate-Free)
• Alternative technique
  arrays, kits
• Alternative to gene target
  v-kit Hardy-Zuckerman 4 feline sarcoma viral oncogene homolog, C-Kit and CD117 and PBT and SCFR, KIT and IDBG-18980 and ENSG00000157404 and 3815, transferase activity, Extracellular, Kit and IDBG-172083 and ENSMUSG00000005672 and 16590, KIT and IDBG-642326 and ENSBTAG00000002699 and 280832

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