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Mouse IgE (epsilon) (min X Hu IgG & M, Ch,Rb,Gt,Sh,Bv,Hrs,Dg,Sw,Bab IgG), Clone: LOME2, Rat Mouse Monoclonal antibody-, Biotin

  • Catalog number
    YULLOME2-B05
  • Product name
    Mouse IgE (epsilon) (min X Hu IgG & M, Ch,Rb,Gt,Sh,Bv,Hrs,Dg,Sw,Bab IgG), Clone: LOME2, Rat Mouse Monoclonal antibody-, Biotin
  • Size
    500ul
  • Price
    618.52
  • Supplier
    accurate-monoclonals
  • Alternative name 1
    Mouse IgE (epsilon) (min X Hu IgG & M, Ch,Rb,Gt,Sh,Bv,Hrs,Dg,Sw,Bab IgG), Clone: LOME2, Rat Mab anti-, Biotin
  • Alternative name 2
    Mouse IgE (epsilon) (min X Hu IgG & M, Ch,Rb,Gt,Sh,Bv,Hrs,Dg,Sw,Bab IgG), Clone: LOME2, Rat Mab antibody-, Biotin
  • Alternative name 3
    Mus musculus IgE (epsilon) (min X Hu IgG & M, Ch,Rb,Gt,Sh,Bv,Hrs,Dg,Sw,Bab IgG), Clone: LOME2, Rattus norvegicus Mab antibody-, Biotin
  • Alternative name 4
    anti- IgE epsilon min X Hu IgG & M Ch Rb Gt Sh Bv Hrs Dg Sw Bab IgG Clone: LOME2
  • Alternative name 5
    IgE epsilon min X Hu IgG & M Ch Rb Gt Sh Bv Hrs Dg Sw Bab IgG Clone: LOME2
  • Clonality
    Monoclonal Antibody
  • Description
    This is a monoclonal antibody which is greatly purified and with high binding affinity for the antigen that it is risen against. If used correctly and according the protocol, this antibody will provide excellent and reproducible results with guaranteed success for the tested and confirmed applications. Amongst the advantages of the monoclonal antibodies are: the fact that while the hybridoma takes a bit longer to be produced, once the line is ready there is virtually an endless supply of these antibodie throughout time with little to no variations in recognition sites of the antigen in different batches; in comparison to the polyclonal antibodies monoclonal antibodies are highly specific to a given epitope and can be used in applications where specific targeting is required.
  • Storage and handling
    Generally, antibodies that are lyophilized can be transported at ambient temperature and stored for short terms at +4 degrees Celsius, for longer periods - at -20 . Antibodies in a liquid form can be shipped and stored for a short period of time at +4 degrees Celsius, for long term storage (up to one year) 25-50% glycerol or ethylene glycol should be added and then the container has to be stored at -20°C.
  • Advisory
    To keep the quality and the affinity of the antibody cycles of freezing and thawing should be avoided. For antibodies in a liquid form or reconstituted lyophilized antibodies small amounts of the soultion could be captured on the cap or the walls of the container. Right before use you could briefly centrifuge the vial to collect all of the solution on the bottom.
  • Properties
    If you buy Antibodies supplied by accurate-monoclonals they should be stored frozen at - 24°C for long term storage and for short term at + 5°C. Biotin conjugates can be detected by horseradish peroxidase, alkaline phosphatase substrates or anti biotin conjugated antibodies. Avidin and Streptavidin bind to the small biotin and are couple to HRP or AP for ELISA. To break the streptavidin Biotin bond we suggest to use a 6 molar guanidine HCl solution with acidity of pH 1.6. Immunoglobulin E (IgE) kappa or Fc specific antibody is a kind of antibody (or immunoglobulin (Ig) "isotype") that has only been found in mammals. IgE is synthesized by plasma cells. Monomers of IgE consist of two heavy chains (ε chain) and two light chains, with the ε chain containing 4 Ig-like constant domains (Cε1-Cε4). IgEs play a role in allergy and response to parasite infection. High levels of IgEs caused by parasites can lower the allergic reaction of patients to allergens common present in the human environment. As such the parasite history of an allergic patient needs to be taken in consideration as a positive factor.
  • Conjugation
    Biotinylated
  • Additional isotype
    IgE, IgG
  • About
    Immunoglobulin gamma, IgG, mouse monoclonal H&L chain clones or rabbit, goat polyclonal antibodies have 4 parts. There are 2 heavy chains, 2 light chains. The IgG antibody has 2 antigen binding sites. They represent 70% or more of serum antibodies. This antibody can be antigen purified or protein A or G purified. For storage sodium azide is added or you can call us to request azide free antibody preparations. These will need colder storage temperatures. Monoclonals of this antigen are available in different clones. Each murine monoclonal anibody has his own affinity specific for the clone. Mouse monoclonal antibodies are purified protein A or G and can be conjugated to FITC for flow cytometry or FACS and can be of different isotypes. Rats are used to make rat monoclonal anti mouse antibodies. There are less rat- than mouse clones however. Rats genes from rodents of the genus Rattus norvegicus are often studied in vivo as a model of human genes in Sprague-Dawley or Wistar rats.
  • Test
    Mouse or mice from the Mus musculus species are used for production of mouse monoclonal antibodies or mabs and as research model for humans in your lab. Mouse are mature after 40 days for females and 55 days for males. The female mice are pregnant only 20 days and can give birth to 10 litters of 6-8 mice a year. Transgenic, knock-out, congenic and inbread strains are known for C57BL/6, A/J, BALB/c, SCID while the CD-1 is outbred as strain.
  • Latin name
    Mus musculus, Rattus norvegicus
  • French translation
    anticorps
  • Gene target
  • Short name
    Mouse IgE (epsilon) (min X Hu IgG M, Ch,Rb,Gt,Sh,Bv,Hrs,Dg,Sw,Bab IgG) Clone: LOME2, Mouse Monoclonal antibody-
  • Technique
    Mouse monoclonal, antibodies against human proteins, antibodies for, IgEs, IgGs, Monoclonals or monoclonal antibodies, mouses, mouse monoclonals
  • Host
    mouse, Murine monoclonal antibodies or Mabs are often conjugated and the isotype is IgG.
  • Isotype
    IgG
  • Label
    Biotin
  • Species
    Mouse, Mouses, Rats
  • Alternative name
    Mouse immunoglobuline E (epsilon) (min X Hu Immunoglobulin G & M, Ch,Rb,Gt,Sh,Bv,Hrs,Dg,Sw,Bab Immunoglobulin G), clonality: LOME2, Rat Mouse monoclonal (Antibody to)-, biotinilated
  • Alternative technique
    antibodies
MeSH Data
  • Name
  • Concept
    Scope note:Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
  • Tree numbers
    • E05.196.401.143
    • E05.301.300.096
    • E05.478.566.320.200
    • E05.601.262
    • E05.601.470.320.200
  • Qualifiers
    classification, economics, history, instrumentation, methods, standards, trends, utilization, veterinary, statistics & numerical data, ethics
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