Mouse anti HLA-DR antibody conjugated to FITC
-
Catalog number0DR2
-
PricePlease ask
-
Size100 Tests
-
-
CategoryPrimary Antibodies
-
Long descriptionHLA-DR is used for the identification of human B cell and T cell subsets associated with approximately 10% of peripheral blood lymphocytes 28-34,000 M.W. surface antigen, and is present in low density on monocytes and macrophages.
-
Antibody come fromHLA-DR=Derived from the hybridization of mouse NS-1/1-Ag4 myeloma cells with spleen cells of BALB/c mice immunized with human lymphoblastoid B-cell line RPMI 8866
-
Other descriptionProvided as sterile filtered solution in phosphate buffered saline with 0.08% sodium azide and 0.2% carrier protein. Protein A/G Chromatography
-
Clone423L
-
Antigen antibody binding interactionMouse anti HLA-DR antibody conjugated to FITC Antibody
-
Antibody is raised inMouse
-
Antibody s reacts withsee techfile
-
Antibody s reacts with these speciesThis antibody doesn't cross react with other species
-
Antibody s specificityNo Data Available
-
ApplicationFlow Cytometry
-
Antibody s suited forPBMC: Add 10 µl of MAB/10^6 PBMC in 100 µl PBS. Mix gently and incubate for 15 minutes at 2¼ to 8¼C. Wash twice with PBS and analyze or fix with 0.5% v/v of paraformaldehyde in PBS and analyze. _x000B_WHOLE BLOOD: Add10 µl of MAB/100 µl of whole blood. Mix gently and incubate for 15 minutes at room temperature 20¼C. Lyse the whole blood. Wash once with PBS and analyze or fix with 0.5% v/v of paraformaldehyde in PBS and analyze. See instrument manufacturerÕs instructions for Lysed Whole Blood and Immunofluorescence analysis with a flow cytometer or microscope.
-
Storage4ºC
-
Relevant references1. IL-4 and granulocyte-macrophage colony-stimulating factor selectively increase HLA-DR and HLA-DP antigens but not HLA-DQ antigens on human monocytes. Gerrard T.L., Dyer D.R., Mostowski H.S., J. Immunol. 1990 June, 1;144(12): 4670-4. _x000B__x000B_2. Structural requirements for pairing of alpha and beta chains in HLA-DR and HLA-DP molecules. Karp D.R., Teletski C.L., Jaraquemada D., Maloy W.L., Coligan J.E., Long E.O., J. Exp.. Med. 1990 March ;171(3):615-28. _x000B__x000B_3. Trans-activation of HLA-DR gene by hepatitis B virus X gene product. Hu K.Q., Vierling J.M., Siddiqui A., Proc. Natl. Acad. Sci. USA 1990 SEPT.; 87(18):7140-4. _x000B__x000B_4. Purified primitive human hematopoietic progenitor cells with long-term in vitro repopulating capacity adhere selectively to irradiated bone marrow stroma. Verfaillie C., Blakolmer K., McGlave P., J. Exp. Med. 1990 Aug.: 172(2):509-2. _x000B__x000B_5. Defective clonogenic potential of CD8+ T lymphocytes in patients with AIDS. Expansion in vivo of a nonclonogenic CD3+ CD8+ DR+ CD25- T cell population. Pantaleo G., Keonig S., Baseler M., Lane H.C., Fauci A.S., J. Immunol. 1990 Mar ; 144(5):1696-704. _x000B__x000B_6. Increased circulating HLA-DR+ CD4+ T cells in systemic lupus erythematosus: alterations associated with prednisolone therapy. Raziuddin S., Nur M.A., al-Wabel A.A., Scand. J. Immunol. 1990 Feb.:31(2):139-45.
-
Protein numbersee ncbi
-
WarningsThis product is intended FOR RESEARCH USE ONLY, and FOR TESTS IN VITRO, not for use in diagnostic or therapeutic procedures involving humans or animals. This datasheet is as accurate as reasonably achievable, but Nordic-MUbio accepts no liability for any inaccuracies or omissions in this information.
-
DescriptionThis antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided.
-
PropertiesIf you buy Antibodies supplied by nordc they should be stored frozen at - 24°C for long term storage and for short term at + 5°C. This nordc Fluorescein isothiocyanate (FITC) antibody is currently after some BD antibodies the most commonly used fluorescent dye for FACS. When excited at 488 nanometers, FITC has a green emission that's usually collected at 530 nanometers, the FL1 detector of a FACSCalibur or FACScan. FITC has a high quantum yield (efficiency of energy transfer from absorption to emission fluorescence) and approximately half of the absorbed photons are emitted as fluorescent light. For fluorescent microscopy applications, the 1 FITC is seldom used as it photo bleaches rather quickly though in flow cytometry applications, its photo bleaching effects are not observed due to a very brief interaction at the laser intercept. nordc FITC is highly sensitive to pH extremes.
-
ConjugationAnti-FITC Antibody
-
TestMouse or mice from the Mus musculus species are used for production of mouse monoclonal antibodies or mabs and as research model for humans in your lab. Mouse are mature after 40 days for females and 55 days for males. The female mice are pregnant only 20 days and can give birth to 10 litters of 6-8 mice a year. Transgenic, knock-out, congenic and inbread strains are known for C57BL/6, A/J, BALB/c, SCID while the CD-1 is outbred as strain.
-
Latin nameMus musculus
-
French translationanticorps
-
Gene target
-
Gene symbolHLA-DPA2, HLA-DOA, HLA-DQA1, HLA-N, HLA-DQA2, HLA-DPB1, HLA-DQB2, HLA-W, HLA-DRA, HLA-S
-
Short nameMouse anti HLA-DR antibody conjugated FITC
-
TechniqueAntibody, Mouse, anti, FITC, antibody to, antibodies against human proteins, antibodies for, antibody Conjugates, Fluorescein, mouses
-
Hostmouse
-
IsotypeIgG2a
-
LabelFITC
-
SpeciesMouse, Mouses
-
Alternative nameMouse antibody to Human leukocyte antigen-DR (antibody to-) coupled to fluorecein
-
Alternative techniqueantibodies, murine, fluorescine
-
Gene info
-
Identity
-
Gene
-
Long gene namemajor histocompatibility complex, class II, DP alpha 2 (pseudogene)
-
Synonyms gene
-
GenBank acession
-
Locus
-
Discovery year1986-01-01
-
Entrez gene record
-
Pubmed identfication
-
Classification
- Histocompatibility complex
-
VEGA ID
-
Locus Specific Databases
Gene info
-
Identity
-
Gene
-
Long gene namemajor histocompatibility complex, class II, DO alpha
-
Synonyms gene
-
Synonyms
-
GenBank acession
-
Locus
-
Discovery year2001-06-22
-
Entrez gene record
-
Pubmed identfication
-
RefSeq identity
-
Classification
- C1-set domain containing
- Histocompatibility complex
-
VEGA ID
-
Locus Specific Databases
Gene info
-
Identity
-
Gene
-
Long gene namemajor histocompatibility complex, class II, DQ alpha 1
-
Synonyms gene
-
Synonyms
-
Locus
-
Discovery year2001-06-22
-
Entrez gene record
-
RefSeq identity
-
Classification
- C1-set domain containing
- Histocompatibility complex
-
VEGA ID
-
Locus Specific Databases
Gene info
-
Identity
-
Gene
-
Long gene namemajor histocompatibility complex, class I, N (pseudogene)
-
Synonyms
-
GenBank acession
-
Locus
-
Discovery year2002-10-23
-
Entrez gene record
-
Pubmed identfication
-
RefSeq identity
-
Classification
- Histocompatibility complex
-
VEGA ID
-
Locus Specific Databases
Gene info
-
Identity
-
Gene
-
Long gene namemajor histocompatibility complex, class II, DQ alpha 2
-
Synonyms gene
-
Locus
-
Discovery year2001-06-22
-
Entrez gene record
-
RefSeq identity
-
Classification
- C1-set domain containing
- Histocompatibility complex
-
VEGA ID
-
Locus Specific Databases
Gene info
-
Identity
-
Gene
-
Long gene namemajor histocompatibility complex, class II, DP beta 1
-
Synonyms gene
-
Locus
-
Discovery year2001-06-22
-
Entrez gene record
-
RefSeq identity
-
Classification
- C1-set domain containing
- Histocompatibility complex
-
VEGA ID
-
Locus Specific Databases
Gene info
-
Identity
-
Gene
-
Long gene namemajor histocompatibility complex, class II, DQ beta 2
-
Synonyms gene
-
GenBank acession
-
Locus
-
Discovery year2001-06-22
-
Entrez gene record
-
Pubmed identfication
-
Classification
- C1-set domain containing
- Histocompatibility complex
-
VEGA ID
-
Locus Specific Databases
Gene info
-
Identity
-
Gene
-
Long gene namemajor histocompatibility complex, class I, W (pseudogene)
-
Synonyms gene
-
Synonyms gene name
- HLA-80 pseudogene
-
GenBank acession
-
Locus
-
Discovery year2004-01-16
-
Entrez gene record
-
Pubmed identfication
-
RefSeq identity
-
Classification
- Histocompatibility complex
-
VEGA ID
-
Locus Specific Databases
Gene info
-
Identity
-
Gene
-
Long gene namemajor histocompatibility complex, class II, DR alpha
-
Synonyms gene
-
Locus
-
Discovery year2001-06-22
-
Entrez gene record
-
RefSeq identity
-
Classification
- C1-set domain containing
- Histocompatibility complex
-
VEGA ID
-
Locus Specific Databases
Gene info
-
Identity
-
Gene
-
Long gene namemajor histocompatibility complex, class I, S (pseudogene)
-
Synonyms
-
GenBank acession
-
Locus
-
Discovery year2002-10-23
-
Entrez gene record
-
Pubmed identfication
-
RefSeq identity
-
Classification
- Histocompatibility complex
-
VEGA ID
-
Locus Specific Databases
MeSH Data
-
Name
-
ConceptScope note: Testing erythrocytes to determine presence or absence of blood-group antigens, testing of serum to determine the presence or absence of antibodies to these antigens, and selecting biocompatible blood by crossmatching samples from the donor against samples from the recipient. Crossmatching is performed prior to transfusion.
-
Tree numbers
- E01.370.225.625.120
- E01.370.225.812.385.120
- E05.200.625.120
- E05.200.812.385.120
- E05.478.594.385.120
-
Qualifiersethics, mortality, psychology, trends, veterinary, history, classification, economics, instrumentation, methods, nursing, standards, adverse effects, statistics & numerical data