Mouse anti CD22 antibody conjugated to FITC
-
Catalog number
0222
-
Price
Please ask
-
Size
100 Tests
-
-
Category
Primary Antibodies
-
Long description
Identification of human B cells associated with approximately 10% of peripheral blood lymphocytes.
-
Antibody come from
n/a
-
Other description
Provided as sterile filtered solution in phosphate buffered saline with 0.08% sodium azide and 0.2% carrier protein. Protein A/G Chromatography
-
Clone
5.8HK
-
Antigen antibody binding interaction
Mouse anti CD22 antibody conjugated to FITC Antibody
-
Antibody is raised in
Mouse
-
Antibody s reacts with
Human
-
Antibody s reacts with these species
This antibody doesn't cross react with other species
-
Antibody s specificity
No Data Available
-
Research interest
CD Marker
-
Application
Flow Cytometry
-
Antibody s suited for
PBMC: Add10 µl of MAB/10^6 PBMC in 100 µl PBS. Mix gently and incubate for 15 minutes at 2¼ to 8¼C. Wash twice with PBS and analyze or fix with 0.5% v/v of paraformaldehyde in PBS and analyze. WHOLE BLOOD: Add 10 µl of MAB/100 µl of whole blood. Mix gently and incubate for 15 minutes at room temperature (20¼C). Lyse the whole blood. Wash once with PBS and analyze or fix with 0.5% v/v of paraformaldehyde in PBS and analyze. See instrument manufacturerÕs instructions for Lysed Whole Blood and Immunofluorescence analysis with a flow cytometer or microscope.
-
Storage
4ºC
-
Relevant references
1. In vivo and in vitro expression of myeloid antigens on B-lineage acute lymphoblastic leukemia cells. Leukemia 1991 Ja;5(1):1925 Hara J; Kawa-Ha K; Yumura-Yagi K; Kurahashi H; Tawa A; Ishihara S; Inoue M; Murayama N; Okada S _x000B__x000B_2. Activation of dense human tonsilar B cells. Induction of c-myc gene expression via two distinct signal transduction pathways. J Immunol 1991 Feb;146(3):846-53 White MW; McConnell F; Shu GL; Morris DR; Clark EA _x000B__x000B_3. Differential effects of low and high concentrations of interleukin 6 on human B cells. Eur J Immunol 1990 No;20(11):2389-93 Levy Y; Fermand JP; Brouet JC _x000B__x000B_4. Immunophenotypes in 'classical' B-cell chronic lymphocytic leukemia. Correlation with normal cellular counterpart and clinical findings. Cancer 1990 Oct 1;66(8):1738-42 Baldini L; Cro L; Cortelezzi A; Calori R; Nobili L; Maiolo AT; Polli EE _x000B__x000B_5. B-cell differentiation following autologous, conventional, or T-cell depleted bone marrow transplantation: a recapitulation of normal B-cell ontogeny. Blood 1990 Oct 1;76(8):1647-56 Small TN; Keever CA; Weiner-Fedus S; Heller G; O'Reilly RJ; Flomenberg N _x000B__x000B_6. Phenotypic analysis of a large number of normal human bone marrow sample by flow cytometry. Blut 1990 No;61(5):271-7 Andreoni C; Rigal D; Bonnard M; Bernaud J _x000B__x000B_7. Identification and characterization of plasma cells in normal human bone marrow by high-resolution flow cytometry. Blood 1990 Nov ;76(9):1739-47 Terstappen LW; Johnsen S; Segers-Nolten IM; Loken MR.
-
Protein number
see ncbi
-
Warnings
This product is intended FOR RESEARCH USE ONLY, and FOR TESTS IN VITRO, not for use in diagnostic or therapeutic procedures involving humans or animals. This datasheet is as accurate as reasonably achievable, but Nordic-MUbio accepts no liability for any inaccuracies or omissions in this information.
-
-
Description
This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided.
-
Properties
If you buy Antibodies supplied by nordc they should be stored frozen at - 24°C for long term storage and for short term at + 5°C. This nordc Fluorescein isothiocyanate (FITC) antibody is currently after some BD antibodies the most commonly used fluorescent dye for FACS. When excited at 488 nanometers, FITC has a green emission that's usually collected at 530 nanometers, the FL1 detector of a FACSCalibur or FACScan. FITC has a high quantum yield (efficiency of energy transfer from absorption to emission fluorescence) and approximately half of the absorbed photons are emitted as fluorescent light. For fluorescent microscopy applications, the 1 FITC is seldom used as it photo bleaches rather quickly though in flow cytometry applications, its photo bleaching effects are not observed due to a very brief interaction at the laser intercept. nordc FITC is highly sensitive to pH extremes.
-
Conjugation
Anti-FITC Antibody
-
Test
Mouse or mice from the Mus musculus species are used for production of mouse monoclonal antibodies or mabs and as research model for humans in your lab. Mouse are mature after 40 days for females and 55 days for males. The female mice are pregnant only 20 days and can give birth to 10 litters of 6-8 mice a year. Transgenic, knock-out, congenic and inbread strains are known for C57BL/6, A/J, BALB/c, SCID while the CD-1 is outbred as strain.
-
Latin name
Mus musculus
-
French translation
anticorps
-
Gene target
-
Gene symbol
CD22
-
Short name
Mouse anti CD22 antibody conjugated FITC
-
Technique
Antibody, Mouse, anti, FITC, antibody to, antibodies against human proteins, antibodies for, antibody Conjugates, Fluorescein, mouses
-
Host
mouse
-
Isotype
IgG2b
-
Label
FITC
-
Species
Mouse, Mouses
-
Alternative name
Mouse antibody to CD22 molecule (antibody to-) coupled to fluorecein
-
Alternative technique
antibodies, murine, fluorescine
-
Alternative to gene target
CD22 molecule, SIGLEC-2 and SIGLEC2, CD22 and IDBG-44001 and ENSG00000012124 and 933, protein binding, Cell surfaces, Cd22 and IDBG-171574 and ENSMUSG00000030577 and 12483, CD22 and IDBG-635610 and ENSBTAG00000011887 and 514582
-
Gene info
-
Identity
-
Gene
-
Long gene name
CD22 molecule
-
Synonyms gene name
-
Synonyms
-
Synonyms name
-
GenBank acession
-
Locus
-
Discovery year
1991-06-06
-
Entrez gene record
-
Pubmed identfication
-
RefSeq identity
-
Classification
- CD molecules
- MicroRNA protein coding host genes
- C2-set domain containing
- Sialic acid binding Ig like lectins
-
VEGA ID
MeSH Data
-
Name
-
Concept
Scope note:
A method for the study of certain organic compounds within cells, in situ, by measuring the light intensities of the selectively stained areas of cytoplasm. The compounds studied and their locations in the cells are made to fluoresce and are observed under a microscope.
-
Tree numbers
- E01.370.225.500.386
- E05.196.712.516.600.240
- E05.200.500.386
- E05.242.386
-
Qualifiers
ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data
Product images
Similar products
