-
Category
Antibody
-
Concentration
In 1X PBS, pH 7.4, with 0.09% sodium azide
-
Form
Purified
-
Conjugation
Unconjugated
-
Clone
Polyclonal antibody
-
Recognised antigen
MYLK3
-
Host animal
Rabbit (Oryctolagus cuniculus)
-
Clonality
Polyclonal (rabbit origin)
-
Species reactivity
Human (Homo sapiens), Mouse (Mus musculus) ; Due to limited knowledge and inability to test the antibody against all known species, we cannot guarantee that no other cross reactivity can occur.
-
Tested applications
IHC, WB, ELISA
-
Recommended dilutions
Western blot: 1:1000,IHC (Paraffin): 1:50-1:100
-
Notes
Titration of the MYLK3 antibody may be required due to differences in protocols and secondary/substrate sensitivity.
-
Intented use
This MYLK3 antibodyis to be used only for research purposes and not for diagnostics..
-
Uniprot
Q32MK0
-
Purity
Purified
-
Description
MLCK/MYLK3, a member of the Ser/Thr protein kinase family, is a calcium/calmodulin-dependent enzyme responsible for smooth muscle contraction via phosphorylation of a specific serine in the N-terminus of myosin light chains (MLC), an event that facilitates myosin interaction with actin filaments. It is a central determinant in the development of vascular permeability and tissue edema formation. In the nervous system it has been shown to control the growth initiation of astrocytic processes in culture and to participate in transmitter release at synapses formed between cultured sympathetic ganglion cells. MLCK acts as a critical participant in signaling sequences that result in fibroblast apoptosis. Smooth muscle and non-muscle isozymes are expressed in a wide variety of adult and fetal tissues and in cultured endothelium with qualitative expression appearing to be neither tissue- nor development-specific. Non-muscle isoform 2 is the dominant splice variant expressed in various tissues. The Telokin isoform, which binds calmodulin, has been found in a wide variety of adult and fetal tissues. MLCK is probably down-regulated by phosphorylation. The protein contains 1 fibronectin type III domain and 9 immunoglobulin-like C2-type domains.
-
Immunogen
A portion of amino acids 40-69 from the human protein was used as the immunogen for this MYLK3 antibody.
-
Storage
Aliquot the MYLK3 antibody and store frozen at -20 deg. Celcius or lower. Cycles of freezing and thawing can denaturate the peptide chains of the antibodies and reduce their sensitivity and/or change their affinity. Prepare aliqotes in such a manner so that freeze-thaw cycles are minimized.
-
Properties
If you buy Antibodies supplied by NJS poly they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.
-
French translation
anticorps
