Hsc70 Antibody

  • Catalog number
    R30315
  • Product name
    Hsc70 Antibody
  • Size
    0.1mg
  • Price
    459.25
  • Supplier
    NJS poly
  • Category
    Antibody
  • Concentration
    0.5mg/ml if reconstituted with 0.2ml sterile DI water
  • Form
    Antigen affinity purified
  • Conjugation
    Unconjugated
  • Clone
    Polyclonal antibody
  • Recognised antigen
    Hsc70
  • Host animal
    Rabbit (Oryctolagus cuniculus)
  • Clonality
    Polyclonal (rabbit origin)
  • Species reactivity
    Human (Homo sapiens), Mouse (Mus musculus), Rat ; Due to limited knowledge and inability to test the antibody against all known species, we cannot guarantee that no other cross reactivity can occur.
  • Tested applications
    WB, IHC-P, IHC-F, ICC
  • Recommended dilutions
    Western blot: 0.5-1ug/ml,IHC (Paraffin): 0.5-1ug/ml,IHC (Frozen): 0.5-1ug/ml,Immunocytochemistry: 0.5-1ug/ml
  • Notes
    The stated application concentrations are suggested starting amounts. Titration of the Hsc70 antibody may be required due to differences in protocols and secondary/substrate sensitivity.
  • Intented use
    This Hsc70 antibodyis to be used only for research purposes and not for diagnostics..
  • Uniprot #
    E9PSH5
  • Purity
    Antigen affinity
  • Description
    The 70 kilodalton heat shock proteins(Hsp70s) are a family of ubiquitously expressed heat shock proteins. The Hsp70s are an important part of the cell's machinery for protein folding, and help to protect cells from stress. All of the Hsp70 proteins have three major functional domains: An N-terminal ATPase domain binds ATP(Adenosine triphosphate) and hydrolyzes it to ADP(Adenosine diphosphate); A substrate binding domain contains a groove with an affinity for neutral, hydrophobic amino acid residues; A C-terminal domain rich in alpha helical structure acts as a 'lid' for the substrate binding domain. By binding tightly to partially-synthesized peptide sequences(incomplete proteins), Hsp70 prevents them from aggregating and being rendered nonfunctional. And it also can act to protect cells from thermal or oxidative stress. Finally, Hsp70 seems to be able to participate in disposal of damaged or defective proteins. Interaction with CHIP(Carboxyl-terminus of Hsp70 Interacting Protein)–an E3 ubiquitin ligase–allows Hsp70 to pass proteins to the cell's ubiquitination and proteolysis pathways.
  • Immunogen
    An amino acid sequence from the N-terminus of human Hsc70 (TTYSCVGVFQHGKVEIIAN) was used as the immunogen for this Hsc70 antibody (100% homologous in human, mouse and rat).
  • Storage
    After reconstitution, the Hsc70 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20 deg. Celcius or lower. Cycles of freezing and thawing can denaturate the peptide chains of the antibodies and reduce their sensitivity and/or change their affinity. Prepare aliqotes in such a manner so that freeze-thaw cycles are minimized. Avoid repeated freezing and thawing.
  • group_crc32
    3828023734
  • Properties
    If you buy Antibodies supplied by NJS poly they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.
  • French translation
    anticorps
  • Gene target
  • Short name
    Hsc70 Antibody
  • Technique
    Antibody, antibodies against human proteins, antibodies for
  • Alternative name
    Hsc70 (Antibody to)
  • Alternative technique
    antibodies
1. Gene info
  • Identity
  • Gene
  • Long gene name
    heat shock protein family A (Hsp70) member 8
  • Synonyms gene
  • Synonyms gene name
    • heat shock 70kD protein 8
    • heat shock 70kDa protein 8
  • Synonyms
  • GenBank acession
  • Locus
  • Discovery year
    1995-05-08
  • Entrez gene record
  • Pubmed identfication
  • Classification
    • Heat shock 70kDa proteins
    • NineTeen complex
  • Havana BLAST/BLAT
MeSH Data
  • Name
  • Concept
    Scope note:Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
  • Tree numbers
    • E05.196.401.143
    • E05.301.300.096
    • E05.478.566.320.200
    • E05.601.262
    • E05.601.470.320.200
  • Qualifiers
    classification, economics, history, instrumentation, methods, standards, trends, utilization, veterinary, statistics & numerical data, ethics
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