Gli1 Antibody

• Catalog number
  R31533
• Price
  Please ask
• Size
  0.1mg

• Category
  Antibody
• Concentration
  0.5mg/ml if reconstituted with 0.2ml sterile DI water
• Form
  Antigen affinity purified
• Conjugation
  Unconjugated
• Clone
  Polyclonal antibody
• Recognised antigen
  Gli1
• Host animal
  Rabbit (Oryctolagus cuniculus)
• Clonality
  Polyclonal (rabbit origin)
• Species reactivity
  Human (Homo sapiens) ; Due to limited knowledge and inability to test the antibody against all known species, we cannot guarantee that no other cross reactivity can occur.
• Tested applications
  WB, IHC-P
• Recommended dilutions
  Western blot: 0.5-1ug/ml,IHC (Paraffin): 0.5-1ug/ml
• Notes
  The stated application concentrations are suggested starting amounts. Titration of the Gli1 antibody may be required due to differences in protocols and secondary/substrate sensitivity.
• Intented use
  This Gli1 antibodyis to be used only for research purposes and not for diagnostics..
• Gene ID
  2735
• Purity
  Antigen affinity
• Description
  Gli1, also known as Gli, is a protein originally isolated in human glioblastoma. The protein is encoded by GLI1 gene. This gene is a member of a select group of cellular genes that are genetically altered in primary human tumors. GLI1 encodes a member of the GLI-Kruppel family of zinc finger proteins in the final steps of Hedgehog signaling in normal development and disease. The encoded transcription factor is activated by the sonic hedgehog signal transduction cascade and regulates stem cell proliferation. The activity and nuclear localization of this protein is negatively regulated by p53 in an inhibitory loop. Beside, various upstream patterning signals may be integrated by the Gli proteins to direct a coherent programme of neurogenesis.
• Immunogen
  Human partial recombinant protein (AA 768-1106) was used as the immunogen for this Gli1 antibody.
• Storage
  After reconstitution, the Gli1 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20 deg. Celcius or lower. Cycles of freezing and thawing can denaturate the peptide chains of the antibodies and reduce their sensitivity and/or change their affinity. Prepare aliqotes in such a manner so that freeze-thaw cycles are minimized. Avoid repeated freezing and thawing.
• Properties
  If you buy Antibodies supplied by NJS poly they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.
• French translation
  anticorps

• Gene target
  Gli1  
• Gene symbol
  GLI1
• Short name
  Anti-Gli1
• Technique
  Antibody, antibodies against human proteins, antibodies for
• Isotype
  Rabbit IgG
• Alternative name
  Antibodies to Gli1
• Alternative technique
  antibodies

Gene info

• Identity
  HGNC:4317
• Gene
  GLI1
• Long gene name
  GLI family zinc finger 1
• Synonyms gene
  • GLI
• Synonyms gene name
  • glioma-associated oncogene homolog 1 (zinc finger protein)
  • glioma-associated oncogene family zinc finger 1
• Locus
  12q13.3
• Discovery year
  1986-01-01
• Entrez gene record
  2735
• Pubmed identfication
  • 2850480
• RefSeq identity
  • NM_005269
• Classification
  • Zinc fingers C2H2-type
• VEGA ID
  OTTHUMG00000167332

MeSH Data

• Name
  Blotting, Western
• Concept
  Scope note: Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
• Tree numbers
  • E05.196.401.143
  • E05.301.300.096
  • E05.478.566.320.200
  • E05.601.262
  • E05.601.470.320.200
• Qualifiers
  ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data

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