CDK1 (Tyr15) Antibody, Cy7 Conjugated
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Catalog numberbs-3092R-Cy7
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PricePlease ask
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Size0.1ml
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Long nameCDK1 (Tyr15) Polyclonal Antibody, Cy7 Conjugated
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CategoryConjugated Primary Antibodies
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ConjugationCy7
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Host OrganismRabbit (Oryctolagus cuniculus)
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Target AntigenCDK1 Tyr15
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SpecificityThis is a highly specific antibody against CDK1 Tyr15. This phosphorylation site is homologous in the listed cross reactive species at the specified location.
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ModificationPhosphorylation
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Modification siteTyr15
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ClonalityPolyclonal
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ClonePolyclonal antibody
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Concentration1ug per 1ul
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Immunogen_range5-25/297
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Subcellular locationCytoplasm, Nucleus, Mitochondrion
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SourceKLH conjugated synthetic phosphopeptide derived from human CDK1 around the phosphorylation site of Tyr15 [GT(p-Y)GV]
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Gene ID number983
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Swiss ProtP06493
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Tested applicationsIF(IHC-P)
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Recommended dilutionsIF(IHC-P)(1:50-200)
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CrossreactivityHuman, Mouse, Rat
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Crossreactive species detailsDue to limited amount of testing and knowledge, not every possible cross-reactivity is known.
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Antigen backgroundPlays a key role in the control of the eukaryotic cell cycle by modulating the centrosome cycle as well as mitotic onset; promotes G2-M transition, and regulates G1 progress and G1-S transition via association with multiple interphase cyclins. Required in higher cells for entry into S-phase and mitosis. Phosphorylates PARVA/actopaxin, APC, AMPH, APC, BARD1, Bcl-xL/BCL2L1, BRCA2, CALD1, CASP8, CDC7, CDC20, CDC25A, CDC25C, CC2D1A, CSNK2 proteins/CKII, FZR1/CDH1, CDK7, CEBPB, CHAMP1, DMD/dystrophin, EEF1 proteins/EF-1, EZH2, KIF11/EG5, EGFR, FANCG, FOS, GFAP, GOLGA2/GM130, GRASP1, UBE2A/hHR6A, HIST1H1 proteins/histone H1, HMGA1, HIVEP3/KRC, LMNA, LMNB, LMNC, LBR, LATS1, MAP1B, MAP4, MARCKS, MCM2, MCM4, MKLP1, MYB, NEFH, NFIC, NPC/nuclear pore complex, PITPNM1/NIR2, NPM1, NCL, NUCKS1, NPM1/numatrin, ORC1, PRKAR2A, EEF1E1/p18, EIF3F/p47, p53/TP53, NONO/p54NRB, PAPOLA, PLEC/plectin, RB1, UL40/R2, RAB4A, RAP1GAP, RCC1, RPS6KB1/S6K1, KHDRBS1/SAM68, ESPL1, SKI, BIRC5/survivin, STIP1, TEX14, beta-tubulins, MAPT/TAU, NEDD1, VIM/vimentin, TK1, FOXO1, RUNX1/AML1, SIRT2 and RUNX2. CDK1/CDC2-cyclin-B controls pronuclear union in interphase fertilized eggs. Essential for early stages of embryonic development. During G2 and early mitosis, CDC25A/B/C-mediated dephosphorylation activates CDK1/cyclin complexes which phosphorylate several substrates that trigger at least centrosome separation, Golgi dynamics, nuclear envelope breakdown and chromosome condensation. Once chromosomes are condensed and aligned at the metaphase plate, CDK1 activity is switched off by WEE1- and PKMYT1-mediated phosphorylation to allow sister chromatid separation, chromosome decondensation, reformation of the nuclear envelope and cytokinesis. Inactivated by PKR/EIF2AK2- and WEE1-mediated phosphorylation upon DNA damage to stop cell cycle and genome replication at the G2 checkpoint thus facilitating DNA repair.
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Purification methodThis antibody was purified via Protein A.
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Storage conditionsKeep the antibody in an aqueous buffered solution containing 1% BSA, 50% glycerol and 0.09% sodium azide. Store refrigerated at 2 to 8 degrees Celcius for up to 1 year.
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Excitation Emission743nm/767nm
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SynonymsCDC2; CDC28A; P34CDC2; Cyclin-dependent kinase 1; CDK1; Cell division control protein 2 homolog; Cell division protein kinase 1; p34 protein kinase; CDKN1
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PropertiesIf you buy Antibodies supplied by Bioss Primary Conjugated Antibodies they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.
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ConjugatedThese antibodies are excite for emission at 650 nm and detected at a 676 nm wavelengths.
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Additional conjugationCy7
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French translationanticorps
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Gene target
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Gene symbolCDK1
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Short nameAnti-CDK1 Tyr15
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TechniqueAntibody, antibodies against human proteins, antibodies for, antibody Conjugates
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IsotypeImmunoglobulin G (IgG)
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LabelCy7
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Alternative nameAnti-CDK1 Tyr15 PAb Cy7
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Alternative techniqueantibodies
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Alternative to gene targetcyclin-dependent kinase 1, CDC2 and CDC28A and P34CDC2, CDK1 and IDBG-75344 and ENSG00000170312 and 983, transferase activity, nuclei, Cdk1 and IDBG-160472 and ENSMUSG00000019942 and 12534, CDK1 and IDBG-628960 and ENSBTAG00000010109 and 281061
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Gene info
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Identity
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Gene
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Long gene namecyclin dependent kinase 1
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Synonyms gene
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Synonyms gene name
- cell division cycle 2, G1 to S and G2 to M
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Synonyms
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GenBank acession
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Locus
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Discovery year1986-01-01
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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Classification
- Cyclin dependent kinases
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VEGA ID
MeSH Data
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Name
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ConceptScope note: Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
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Tree numbers
- E05.196.401.143
- E05.301.300.096
- E05.478.566.320.200
- E05.601.262
- E05.601.470.320.200
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Qualifiersethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data