This antibody reacts with:
Synthetic peptide surrounding amino acid 279 of human YAP1
How to handle this antibody:
The antibody solution should be gently mixed before use.
Form of antibody:
This antibody has been succesfully tested for use in following methods:
Target of this antibody:
Formulation of this antibody:
100 µg (0.5 mg/ml) affinity purified rabbit polyclonal antibody in phosphate-buffered saline (PBS) containing 30% glycerol, 0.5% BSA, and 0.01% thimerosal.
Alternative names of antibody target:
YAP-1, YAP1, YAP 1, Yes associated protein 1, YAP65, YAP 65
Western blotting (0.5-4 µg/ml). Jurkat cell lysate can be used as a positive control. Other applications have not been tested.
Shelf time: 12 months in temp.: -20 deg.C
Clonality of antibody:
Yes-associated protein (YAP) was identified based on its ability to associate with the SH3 domain of Yes. It also binds to other SH3 domain containing proteins such as Nck, Crk, Src, and Abl. YAP is a transcriptional coactivator. It interacts with 13-3-3 and suppresses apoptosis.
Concentration of antibody:
This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided.
If you buy Antibodies supplied by biovision antibodies-kits-proteins they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.