This antibody reacts with:
Synthetic peptide of human TIF1α/TRIM24
How to handle this antibody:
The antibody solution should be gently mixed before use.
Form of antibody:
This antibody has been succesfully tested for use in following methods:
Target of this antibody:
Formulation of this antibody:
100 µl affinity purified rabbit polyclonal antibody in phosphate-buffered saline (PBS) containing 30% glycerol, 0.5% BSA and 0.01% thimerosal.
Alternative names of antibody target:
TF1A, TIF1, hTIF1, TIF1A, TIF1 Alpha, Transcriptional Intermediary Factor 1 Alpha, TRIM24, Tripartite Motif-Containing 24, RNF82, Ring Finger Protein 82, PTC6
Western blotting (1:500 – 1:2000). However, the optimal concentrations should be determined individually. HeLa cell lysate can be used as a positive control. The antibody recognizes the TIF1α/TRIM24 of human and mouse origins. Reactivity to other species has not been tested.
Shelf time: 12 months in temp.: -20 deg.C
Clonality of antibody:
TIF1 Alpha/TRIM24 is a member of a family of proteins that control transcription and chromatin remodeling through their interaction with transcription factors. The family of TIF1s (transcriptional intermediary factor 1) includes TIF1-alpha, TIF1-beta, and TIF1-gamma. TIF1-alpha plays an important role in development and mediates transcriptional control by interacting with the AF2 region of nuclear receptors such as estrogen, retinoic acid, and vitamin D3 receptors. TIF1-alpha has been shown to be a repressor of transcription. This transcriptional repression involves histone deacetylation and interaction with members of the heterochromatin protein 1 family.
Concentration of antibody:
This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided.
If you buy Antibodies supplied by biovision antibodies-kits-proteins they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.
1. Gene info
Scope note:Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
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