This antibody reacts with:
Human, mouse, rat
Synthetic peptide surrounding Ser9 of human GSK-3β
How to handle this antibody:
The antibody solution should be gently mixed before use.
Form of antibody:
This antibody has been succesfully tested for use in following methods:
Western blot, Immunohistochemistry
Target of this antibody:
Formulation of this antibody:
100 µg (0.5 mg/ml) immunoaffinity purified rabbit polyclonal antibody in phosphate buffered saline (PBS), pH 7.2, containing 50% glycerol, 1% BSA, 0.02% thimerosal.
Alternative names of antibody target:
EC 184.108.40.206, GSK-3beta , glycogen synthase kinase 3 beta
Western blot analysis (1-2 µg/ml) and Immunohistochemistry (15-30 µg/ml). However, the optimal conditions should be determined individually. The antibody detects phosphorylated GSK-3β (Ser9) in samples from human, mouse, and rat origin. It does not recognize the phosphorylated GSK-3α.
Shelf time: 12 months in temp.: -20 deg.C
Clonality of antibody:
Glycogen synthase kinase 3 (GSK-3) is a serine/threonine protein kinase that has been implicated in the regulation of cell fate and in the Wnt signaling pathway. GSK-3 plays an important role in the PI3 kinase and Akt mediated cell survival pathways, and its activity can be inhibited by Akt-mediated phosphorylation at Ser21 of GSK-3α and Ser9 of GSK-3β. GSK-3 has also been implicated in alzheimer’s disease. Six Tau protein isoforms have been identified, all of which are phosphorylated by GSK-3.
Concentration of antibody:
This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided.
If you buy Antibodies supplied by biovision antibodies-kits-proteins they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.
Scope note:Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
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