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anti-Phospho-GSK-3b Antibody

  • Catalog number
    3495-100
  • Product name
    anti-Phospho-GSK-3b Antibody
  • Size
    Phospho-GSK-3b Antibody
  • Price
    425EUR
  • Supplier
    biovision antibodies-kits-proteins
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  • Antibody host:
    Rabbit
  • This antibody reacts with:
    Human, mouse, rat
  • Purification method:
    Affinity purified
  • Immunogene sequence:
    Synthetic peptide surrounding Ser9 of human GSK-3β
  • How to handle this antibody:
    The antibody solution should be gently mixed before use.
  • Form of antibody:
    Liquid
  • This antibody has been succesfully tested for use in following methods:
    Western blot, Immunohistochemistry
  • Target of this antibody:
    Phospho-GSK-3b
  • Formulation of this antibody:
    100 µg (0.5 mg/ml) immunoaffinity purified rabbit polyclonal antibody in phosphate buffered saline (PBS), pH 7.2, containing 50% glycerol, 1% BSA, 0.02% thimerosal.
  • Alternative names of antibody target:
    EC 2.7.11.26, GSK-3beta , glycogen synthase kinase 3 beta
  • Recommended usage:
    Western blot analysis (1-2 µg/ml) and Immunohistochemistry (15-30 µg/ml). However, the optimal conditions should be determined individually. The antibody detects phosphorylated GSK-3β (Ser9) in samples from human, mouse, and rat origin. It does not recognize the phosphorylated GSK-3α.
  • Storage information:
    Shelf time: 12 months in temp.: -20 deg.C
  • Shipping conditions:
    gel pack
  • Clonality of antibody:
    Polyclonal
  • Isotype:
    Rabbit IgG
  • Background information:
    Glycogen synthase kinase 3 (GSK-3) is a serine/threonine protein kinase that has been implicated in the regulation of cell fate and in the Wnt signaling pathway. GSK-3 plays an important role in the PI3 kinase and Akt mediated cell survival pathways, and its activity can be inhibited by Akt-mediated phosphorylation at Ser21 of GSK-3α and Ser9 of GSK-3β. GSK-3 has also been implicated in alzheimer’s disease. Six Tau protein isoforms have been identified, all of which are phosphorylated by GSK-3.
  • Concentration of antibody:
    0.5 mg/ml
  • Description
    This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided.
  • Properties
    If you buy Antibodies supplied by biovision antibodies-kits-proteins they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.
  • Gene target
  • Short name
    anti-Phospho-GSK-3b Antibody
  • Technique
    Antibody, antibody to, antibodies against human proteins, antibodies for
  • Alternative name
    antibody to-phosphorilated-GSK-3b (Antibody to)
  • Alternative technique
    antibodies
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MESH Data
  • Name
  • Concept
    Scope note: Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
  • Tree numbers
    • E05.196.401.143
    • E05.301.300.096
    • E05.478.566.320.200
    • E05.601.262
    • E05.601.470.320.200
  • Qualifiers
    classification, economics, history, instrumentation, methods, standards, trends, utilization, veterinary, statistics & numerical data, ethics
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