anti-PARP (Cleaved) Antibody
This antibody reacts with:
Synthetic peptide correspond to the N-terminal residues of the catalytic domain of human PARP
How to handle this antibody:
The antibody solution should be gently mixed before use.
Form of antibody:
This antibody has been succesfully tested for use in following methods:
Western blot, Immunohistochemistry
Target of this antibody:
Formulation of this antibody:
100 µg (0.2 mg/ml) affinity purified rabbit polyclonal anti-cleaved PARP antibody in phosphate-buffered saline (PBS) containing 50% glycerol, 1% BSA, and 0.02% thimerosal.
Alternative names of antibody target:
PARP1, ADPRT, PPOL, NAD(+) ADP-ribosyltransferase 1
Western blot analysis (0.5-4 µg/ml) and immunocytochemistry (10-20 µg/ml). However, the optimal concentrations should be determined individually. The antibody recognizes only the large fragment of PARP (89 kDa) and does not react with the full length PARP. Camptothecin treated Jurkat cell lysate can be used as a positive control.
Shelf time: 12 months in temp.: -20 deg.C
Clonality of antibody:
PARP, a 116 kDa nuclear poly (ADP-ribose) polymerase, is a highly conserved nuclear enzyme implicated in DNA repair and in the apoptosis response of cells. This protein can be cleaved by many caspases in vitro and is one of the main cleavage targets of caspase-3 in vivo. The cleavage occurs between ASP214 and Gly 215, which separates PARP’s N-terminal DNA binding domain (24 kDa) from its C-terminal catalytic domain (89 kDa). It has been shown that cleavage of PARP facilitates cellular disassembly and inhibition of PARP cleavage attenuates apoptosis in vitro.
Concentration of antibody:
This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided.
If you buy Antibodies supplied by biovision antibodies-kits-proteins they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.
1. Gene info
Scope note:Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
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