Min length of search pharse is two chars

Filters

anti-PARP (Cleaved) Antibody

  • Catalog number
    3140-100
  • Product name
    anti-PARP (Cleaved) Antibody
  • Size
    PARP (Cleaved) Antibody
  • Price
    525.63
  • Supplier
    biovision antibodies-kits-proteins
  • Antibody host:
    Rabbit
  • This antibody reacts with:
    Human
  • Purification method:
    Affinity purified
  • Immunogene sequence:
    Synthetic peptide correspond to the N-terminal residues of the catalytic domain of human PARP
  • How to handle this antibody:
    The antibody solution should be gently mixed before use.
  • Form of antibody:
    Liquid
  • This antibody has been succesfully tested for use in following methods:
    Western blot, Immunohistochemistry
  • Target of this antibody:
    PARP
  • Formulation of this antibody:
    100 µg (0.2 mg/ml) affinity purified rabbit polyclonal anti-cleaved PARP antibody in phosphate-buffered saline (PBS) containing 50% glycerol, 1% BSA, and 0.02% thimerosal.
  • Alternative names of antibody target:
    PARP1, ADPRT, PPOL, NAD(+) ADP-ribosyltransferase 1
  • Recommended usage:
    Western blot analysis (0.5-4 µg/ml) and immunocytochemistry (10-20 µg/ml). However, the optimal concentrations should be determined individually. The antibody recognizes only the large fragment of PARP (89 kDa) and does not react with the full length PARP. Camptothecin treated Jurkat cell lysate can be used as a positive control.
  • Storage information:
    Shelf time: 12 months in temp.: -20 deg.C
  • Shipping conditions:
    gel pack
  • Clonality of antibody:
    Polyclonal
  • Isotype:
    Rabbit IgG
  • Background information:
    PARP, a 116 kDa nuclear poly (ADP-ribose) polymerase, is a highly conserved nuclear enzyme implicated in DNA repair and in the apoptosis response of cells. This protein can be cleaved by many caspases in vitro and is one of the main cleavage targets of caspase-3 in vivo. The cleavage occurs between ASP214 and Gly 215, which separates PARP’s N-terminal DNA binding domain (24 kDa) from its C-terminal catalytic domain (89 kDa). It has been shown that cleavage of PARP facilitates cellular disassembly and inhibition of PARP cleavage attenuates apoptosis in vitro.
  • Concentration of antibody:
    0.2 mg/ml
  • Description
    This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided.
  • Properties
    If you buy Antibodies supplied by biovision antibodies-kits-proteins they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.
  • Gene target
  • Short name
    anti-PARP (Cleaved) Antibody
  • Technique
    Antibody, antibody to, antibodies against human proteins, antibodies for
  • Alternative name
    antibody to-PARP (Cleaved) (Antibody to)
  • Alternative technique
    antibodies
1. Gene info
  • Identity
  • Gene
  • Long gene name
    poly(ADP-ribose) polymerase 1
  • Synonyms gene
  • Synonyms gene name
    • ADP-ribosyltransferase (NAD+; poly (ADP-ribose) polymerase)
    • poly (ADP-ribose) polymerase family, member 1
  • Synonyms
  • GenBank acession
  • Locus
  • Discovery year
    1986-01-01
  • Entrez gene record
  • Pubmed identfication
  • RefSeq identity
  • Classification
    • Zinc fingers PARP-type
    • Zinc fingers
    • Poly(ADP-ribose) polymerases
  • Havana BLAST/BLAT
MeSH Data
  • Name
  • Concept
    Scope note:Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
  • Tree numbers
    • E05.196.401.143
    • E05.301.300.096
    • E05.478.566.320.200
    • E05.601.262
    • E05.601.470.320.200
  • Qualifiers
    classification, economics, history, instrumentation, methods, standards, trends, utilization, veterinary, statistics & numerical data, ethics
Related products
Chat with gentaur.com employee