This antibody reacts with:
Human, mouse, rat, monkey, hamster, rabbit, bovine
Synthetic peptide surrounding amino acid 559 of human Hsc70
How to handle this antibody:
The antibody solution should be gently mixed before use.
Form of antibody:
This antibody has been succesfully tested for use in following methods:
Western blot, Immunohistochemistry
Target of this antibody:
Formulation of this antibody:
100 µg (0.5 mg/ml) affinity purified rabbit anti-Hsc70 polyclonal antibody in phosphate buffered saline (PBS), pH 7.2, containing 30% glycerol, 0.5% BSA, 0.01% thimerosal.
Alternative names of antibody target:
HSPA8, LAP1, HSC54, HSC70, HSC71, HSP71, HSP73, HSPA10, MGC131511, MGC29929, NIP71
Western blot analysis (0.5-4 µg/ml) and in Immunohistochemistry. However, the optimal conditions should be determined individually. The antibody detects a 71 kDa Hsc70. A ~40 kDa band can also be detected in human samples. Jurkat cell lysate (Cat.# 2401-100) and mouse small intestine lysate can be used as positive controls.
Shelf time: 12 months in temp.: -20 deg.C
Clonality of antibody:
The 70 kDa heat shock cognate protein, Hsc70, is part of the Hsp70 family. The family includes both cognate members and highly inducible isoforms. Members of the Hsp70 family are molecular chaperones which are involved in many cellular functions such as protein folding, transport, maturation and degradation.
Concentration of antibody:
This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided.
If you buy Antibodies supplied by biovision antibodies-kits-proteins they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.