This antibody reacts with:
Synthetic peptide from a portion of human BRG1/SMARCA4 protein
How to handle this antibody:
The antibody solution should be gently mixed before use.
Form of antibody:
This antibody has been succesfully tested for use in following methods:
Target of this antibody:
Formulation of this antibody:
100 µl purified rabbit polyclonal antibody in phosphate-buffered saline (PBS) containing 30% glycerol, 1% BSA and 0.02% thimerosal.
Alternative names of antibody target:
BRG1, BRM/SWI2-Related Gene 1, SMARCA4, SWI/SNF Related, Matrix Associated, Actin Dependent Regulator of Chromatin, Subfamily A, member 4, SNF2L4, Sucrose Nonfermenting 2-Like 4
Western blotting (1:500 – 1:2500 µg/ml). However, the optimal concentrations should be determined individually. HeLa cell lysate can be used as a positive control. The antibody recognizes the BRG1/SMARCA4 of human and mouse origins. Reactivity to other species has not been tested.
Shelf time: 12 months in temp.: -20 deg.C
Clonality of antibody:
The acetylation of histone lysine residues plays a crucial role in the epigenetic regulation of gene transcription. A bromodomain is a protein domain that recognizes acetylated lysine residues such as those on the N-terminal tails of histones. This recognition is often a prerequisite for protein-histone association and chromatin remodeling. These domains function in the linking of protein complexes to acetylated nucleosomes, thereby controlling chromatin structure and gene expression. Thus, bromodomains serve as “readers” of histone acetylation marks regulating the transcription of target promoters. BRG1 is a member of the SWI/SNF protein family, which forms part of a large ATP-dependent chromatin remodeling complex. This complex is required for transcriptional activation of genes normally repressed by chromatin. BRG1 is mutated in many cancer cell lines, such as breast, prostate, lung, pancreas and colon. Further, BRG1 has an important role as a tumor suppressor. This protein can be used for the study of bromodomain binding assays, screening inhibitors, and selectivity profiling.
Concentration of antibody:
This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided.
If you buy Antibodies supplied by biovision antibodies-kits-proteins they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.