This antibody reacts with:
Human, mouse, rat, chicken, rabbit
Synthetic peptide surrounding amino acid 395 of human BMP-4
How to handle this antibody:
The antibody solution should be gently mixed before use.
Form of antibody:
This antibody has been succesfully tested for use in following methods:
Western blot, Immunohistochemistry
Target of this antibody:
Formulation of this antibody:
100 µg (0.5 mg/ml) affinity purified rabbit anti-BMP-4 polyclonal antibody in phosphate buffered saline (PBS), pH 7.2, containing 30% glycerol, 0.5% BSA, and 0.01% thimerosal.
Alternative names of antibody target:
BMP4, BMP-4, BMP 4, bone morphogenetic protein 4, BMP2B, BMP2B1
Western blotting (0.5-4 µg/ml). However, the optimal conditions should be determined individually.
Shelf time: 12 months in temp.: -20 deg.C
Clonality of antibody:
BMPs (bone morphogenetic proteins) belong to the TGF-beta superfamily of structurally related signaling proteins. Members of this superfamily are widely represented throμghout the animal kingdom and have been implicated in a variety of developmental processes. Proteins of the TGF-beta superfamily are disulfide-linked dimmers composed of two 12-15 kDa polypeptide chains. As implied by their name, BMPs initiate, promote and regulate bone development, growth, remodeling and repair. Smad1 translocation to the nucleus is observed after the addition of BMP-4 (also designated BMP-2B), sμggesting that BMP-4 may play a role in activation of the Smad pathway.
Concentration of antibody:
This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided.
If you buy Antibodies supplied by biovision antibodies-kits-proteins they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.