AKT3 Antibody

• Catalog number
  F50045-0.08ML
• Price
  Please ask
• Size
  0.08 ml

• Category
  Antibody
• Concentration
  In 1X PBS, pH 7.4, with 0.09% sodium azide
• Form
  Purified
• Conjugation
  Unconjugated
• Clone
  Polyclonal antibody
• Recognised antigen
  AKT3
• Host animal
  Rabbit (Oryctolagus cuniculus)
• Clonality
  Polyclonal (rabbit origin)
• Species reactivity
  Human (Homo sapiens), Mouse (Mus musculus), Primate, Rat ; Due to limited knowledge and inability to test the antibody against all known species, we cannot guarantee that no other cross reactivity can occur.
• Tested applications
  IHC, WB, ELISA
• Recommended dilutions
  Western blot: 1:1000,IHC (Paraffin): 1:50-1:100
• Notes
  Titration of the AKT3 antibody may be required due to differences in protocols and secondary/substrate sensitivity.
• Intented use
  This AKT3 antibodyis to be used only for research purposes and not for diagnostics..
• Uniprot
  Q9Y243
• Purity
  Purified
• Description
  AKT3 is one of 3 closely related serine/threonine-protein kinases (AKT1, AKT2 and AKT3) called the AKT kinase, and which regulate many processes including metabolism, proliferation, cell survival, growth and angiogenesis. AKT3 is the least studied AKT isoform. It plays an important role in brain development and is crucial for the viability of malignant glioma cells. AKT3 isoform may also be the key molecule in up-regulation and down-regulation of MMP13 via IL13. Required for the coordination of mitochondrial biogenesis with growth factor-induced increases in cellular energy demands. Down-regulation by RNA interference reduces the expression of the phosphorylated form of BAD, resulting in the induction of caspase-dependent apoptosis. [UniProt]
• Immunogen
  A portion of amino acids 88-118 from the human protein was used as the immunogen for this AKT3 antibody.
• Storage
  Aliquot the AKT3 antibody and store frozen at -20 deg. Celcius or lower. Cycles of freezing and thawing can denaturate the peptide chains of the antibodies and reduce their sensitivity and/or change their affinity. Prepare aliqotes in such a manner so that freeze-thaw cycles are minimized.
• Properties
  If you buy Antibodies supplied by NJS poly they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.
• French translation
  anticorps

• Gene target
  AKT3  
• Gene symbol
  AKT3-IT1, AKT3
• Short name
  Anti-AKT3
• Technique
  Antibody, antibodies against human proteins, antibodies for
• Isotype
  Rabbit Ig
• Alternative name
  Antibodies to AKT3
• Alternative technique
  antibodies

Gene info

• Identity
  HGNC:41304
• Gene
  AKT3-IT1
• Long gene name
  AKT3 intronic transcript 1
• Synonyms gene name
  • AKT3 intronic transcript 1 (non-protein coding)
• Locus
  1q44
• Discovery year
  2011-05-20
• Classification
  • Intronic transcripts
• VEGA ID
  OTTHUMG00000039993

Gene info

• Identity
  HGNC:393
• Gene
  AKT3
• Long gene name
  AKT serine/threonine kinase 3
• Synonyms gene name
  • v-akt murine thymoma viral oncogene homolog 3
• Synonyms
  • PKBG
  • RAC-gamma
  • PRKBG
• Synonyms name
  • protein kinase B, gamma
• GenBank acession
  • AF124141
• Locus
  1q43-q44
• Discovery year
  1999-11-16
• Entrez gene record
  10000
• Pubmed identfication
  • 10092583
  • 10208883
• RefSeq identity
  • NM_181690
• Classification
  • Pleckstrin homology domain containing
  • AKT kinases
• VEGA ID
  OTTHUMG00000039994
• Locus Specific Databases
  • LRG_1396

MeSH Data

• Name
  Blotting, Western
• Concept
  Scope note: Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
• Tree numbers
  • E05.196.401.143
  • E05.301.300.096
  • E05.478.566.320.200
  • E05.601.262
  • E05.601.470.320.200
• Qualifiers
  ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data

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