Rat Ly6 A/E (Sca-1) Mouse Monoclonal Antibody [D7]-Biotin
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Catalog numberBD-MOLY6AB(V100)
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Price:
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Size0,1 mg
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DescriptionRat Ly6 A/E (Sca-1) Mouse Monoclonal Antibody [D7]-Biotin is a mouse monoclonal antibody, specific for [D7] clones, this product was conjugated with Biotin format and reported for use in flow cytometric analysis to interacts with Ly6 A/E glycosylphosphatidylinositol linked proteins in hematopoietic stem cells, activated T and B cells, also found on subset of resting B and T cells, Ly6 A/E plays a role of response regulation for T and B cells.
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CloneD7
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ConjugateBiotin
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Gene NameLy6 A/E
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Other Names of geneSca-1
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Concentration500 µg/mL
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ApplicationFlow Cytometry Method
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ImmunogenIL-2-dependent mouse T-cell line CTL-L
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FormulationAqueous buffered solution containing protein stabilizer, and ≤0.09% sodium azide (NaN3).
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Storage2-8°C, store in dark, do not freeze
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ExpirationAfter 1 year
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RemarkFor Recherche Use Only.
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PropertiesIf you buy Antibodies supplied by Immunostep they should be stored frozen at - 24°C for long term storage and for short term at + 5°C. Biotin conjugates can be detected by horseradish peroxidase, alkaline phosphatase substrates or anti biotin conjugated antibodies. Avidin and Streptavidin bind to the small biotin and are couple to HRP or AP for ELISA. To break the streptavidin Biotin bond we suggest to use a 6 molar guanidine HCl solution with acidity of pH 1.6.
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ConjugationBiotinylated
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AboutMonoclonals of this antigen are available in different clones. Each murine monoclonal anibody has his own affinity specific for the clone. Mouse monoclonal antibodies are purified protein A or G and can be conjugated to FITC for flow cytometry or FACS and can be of different isotypes. Rats are used to make rat monoclonal anti mouse antibodies. There are less rat- than mouse clones however. Rats genes from rodents of the genus Rattus norvegicus are often studied in vivo as a model of human genes in Sprague-Dawley or Wistar rats.
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TestMouse or mice from the Mus musculus species are used for production of mouse monoclonal antibodies or mabs and as research model for humans in your lab. Mouse are mature after 40 days for females and 55 days for males. The female mice are pregnant only 20 days and can give birth to 10 litters of 6-8 mice a year. Transgenic, knock-out, congenic and inbread strains are known for C57BL/6, A/J, BALB/c, SCID while the CD-1 is outbred as strain.
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Latin nameMus musculus, Rattus norvegicus
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French translationanticorps
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Gene target
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Short nameLy6 A/E (Sca-1) Mouse Monoclonal Antibody [D7]-
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TechniqueMouse monoclonal, Antibody, Mouse, antibodies against human proteins, antibodies for, Monoclonals or monoclonal antibodies, mouses, mouse monoclonals
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HostRat, Murine monoclonal antibodies or Mabs are often conjugated and the isotype is IgG.
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IsotypeRat IgG2a
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LabelBiotin
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SpeciesMouse, Mouses, Rats
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Alternative nameRat Anti Mouse Ly6 A/E (Sca-1) Antibody-Biotin
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Alternative techniqueantibodies, murine
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MeSH Data
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Name
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ConceptScope note: Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
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Tree numbers
- E05.196.401.143
- E05.301.300.096
- E05.478.566.320.200
- E05.601.262
- E05.601.470.320.200
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Qualifiersethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data