Rat CD8b Mouse Monoclonal Antibody [H35-17.2]-FITC
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Catalog number
BD-MO8BF(V100)
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Price
Please ask
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Size
0,1 mg
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Description
Rat CD8b Mouse Monoclonal Antibody [H35-17.2]-FITC is a mouse monoclonal antibody, specific for [H35-17.2] clones, this product was conjugated with FITC format and reported for use in flow cytometric analysis to react with CD8b proteins found in a large part of thymocytes and subsets of T cells, CD8b function is a TCR co-receptor and activate T cells.
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Clone
H35-17.2
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Conjugate
FITC
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Gene Name
CD8 b
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Other Names of gene
Ly-3, Ly-C, Lyt-3
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Concentration
500 µg/mL
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Application
Flow Cytometry Method
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Immunogen
5-day MLR, C57BL/6 anti-BALB/c
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Formulation
Aqueous buffered solution containing protein stabilizer, and ≤0.09% sodium azide (NaN3).
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Storage
2-8°C, store in dark, do not freeze
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Expiration
After 1 year
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Remark
For Recherche Use Only.
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Properties
If you buy Antibodies supplied by Immunostep they should be stored frozen at - 24°C for long term storage and for short term at + 5°C. This Immunostep Fluorescein isothiocyanate (FITC) antibody is currently after some BD antibodies the most commonly used fluorescent dye for FACS. When excited at 488 nanometers, FITC has a green emission that's usually collected at 530 nanometers, the FL1 detector of a FACSCalibur or FACScan. FITC has a high quantum yield (efficiency of energy transfer from absorption to emission fluorescence) and approximately half of the absorbed photons are emitted as fluorescent light. For fluorescent microscopy applications, the 1 FITC is seldom used as it photo bleaches rather quickly though in flow cytometry applications, its photo bleaching effects are not observed due to a very brief interaction at the laser intercept. Immunostep FITC is highly sensitive to pH extremes.
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Conjugation
Anti-FITC Antibody
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About
Monoclonals of this antigen are available in different clones. Each murine monoclonal anibody has his own affinity specific for the clone. Mouse monoclonal antibodies are purified protein A or G and can be conjugated to FITC for flow cytometry or FACS and can be of different isotypes. Rats are used to make rat monoclonal anti mouse antibodies. There are less rat- than mouse clones however. Rats genes from rodents of the genus Rattus norvegicus are often studied in vivo as a model of human genes in Sprague-Dawley or Wistar rats.
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Test
Mouse or mice from the Mus musculus species are used for production of mouse monoclonal antibodies or mabs and as research model for humans in your lab. Mouse are mature after 40 days for females and 55 days for males. The female mice are pregnant only 20 days and can give birth to 10 litters of 6-8 mice a year. Transgenic, knock-out, congenic and inbread strains are known for C57BL/6, A/J, BALB/c, SCID while the CD-1 is outbred as strain.
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Latin name
Mus musculus, Rattus norvegicus
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French translation
anticorps
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Gene target
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Gene symbol
PIRC95, PIRC91, PIRC90, SNORD115-17, IGHV3OR16-17, PIRC92, PIRC93, SNORD116-17
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Short name
CD8b Mouse Monoclonal Antibody [H35-17 2]-FITC
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Technique
Mouse monoclonal, Antibody, Mouse, FITC, antibodies against human proteins, antibodies for, Fluorescein, Monoclonals or monoclonal antibodies, mouses, mouse monoclonals
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Host
Rat, Murine monoclonal antibodies or Mabs are often conjugated and the isotype is IgG.
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Isotype
Rat Kappa IgG2b
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Label
FITC
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Species
Mouse, Mouses, Rats
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Alternative name
Rat Anti Mouse CD8 b Antibody-FITC
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Alternative technique
antibodies, murine, fluorescine
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Alternative to gene target
CD8b molecule, CD8B and IDBG-60041 and ENSG00000172116 and 100996919,926, MHC class I protein binding, Extracellular, Cd8b1 and IDBG-155547 and ENSMUSG00000053044 and 12526, CD8B and IDBG-634906 and ENSBTAG00000008956 and 508633
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Gene info
Gene info
Gene info
Gene info
Gene info
Gene info
Gene info
Gene info
MeSH Data
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Name
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Concept
Scope note:
A method for the study of certain organic compounds within cells, in situ, by measuring the light intensities of the selectively stained areas of cytoplasm. The compounds studied and their locations in the cells are made to fluoresce and are observed under a microscope.
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Tree numbers
- E01.370.225.500.386
- E05.196.712.516.600.240
- E05.200.500.386
- E05.242.386
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Qualifiers
ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data
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