Mouse H2Db Mouse Monoclonal Antibody [KH95]-FITC
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Catalog number
BD-MOH2DBF(V25)
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Price
Please ask
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Size
0,025 mg
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Description
Mouse H2Db Mouse Monoclonal Antibody [KH95]-FITC is a mouse monoclonal antibody, specific for [KH95] clones, this product was conjugated with FITC format and reported for use in flow cytometric analysis to identify HD2b antigens that found on nucleated cells of the H-2Db haplotype, HD2b function is the presentation of antigens.
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Clone
KH95
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Conjugate
FITC
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Gene Name
HD2b
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Other Names of gene
MHC class I
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Concentration
500 µg/mL
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Application
Flow Cytometry Method
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Immunogen
C57BL/10 mouse skin graft and splenocytes
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Formulation
Aqueous buffered solution containing protein stabilizer, and ≤0.09% sodium azide (NaN3).
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Storage
2-8°C, store in dark, do not freeze
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Expiration
After 1 year
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Remark
For Recherche Use Only.
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Properties
If you buy Antibodies supplied by Immunostep they should be stored frozen at - 24°C for long term storage and for short term at + 5°C. This Immunostep Fluorescein isothiocyanate (FITC) antibody is currently after some BD antibodies the most commonly used fluorescent dye for FACS. When excited at 488 nanometers, FITC has a green emission that's usually collected at 530 nanometers, the FL1 detector of a FACSCalibur or FACScan. FITC has a high quantum yield (efficiency of energy transfer from absorption to emission fluorescence) and approximately half of the absorbed photons are emitted as fluorescent light. For fluorescent microscopy applications, the 1 FITC is seldom used as it photo bleaches rather quickly though in flow cytometry applications, its photo bleaching effects are not observed due to a very brief interaction at the laser intercept. Immunostep FITC is highly sensitive to pH extremes.
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Conjugation
Anti-FITC Antibody
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About
Monoclonals of this antigen are available in different clones. Each murine monoclonal anibody has his own affinity specific for the clone. Mouse monoclonal antibodies are purified protein A or G and can be conjugated to FITC for flow cytometry or FACS and can be of different isotypes.
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Test
Mouse or mice from the Mus musculus species are used for production of mouse monoclonal antibodies or mabs and as research model for humans in your lab. Mouse are mature after 40 days for females and 55 days for males. The female mice are pregnant only 20 days and can give birth to 10 litters of 6-8 mice a year. Transgenic, knock-out, congenic and inbread strains are known for C57BL/6, A/J, BALB/c, SCID while the CD-1 is outbred as strain.
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Latin name
Mus musculus
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French translation
anticorps
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Gene target
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Short name
Mouse H2Db Mouse Monoclonal Antibody [KH95]-FITC
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Technique
Mouse monoclonal, Antibody, Mouse, FITC, antibodies against human proteins, antibodies for, Fluorescein, Monoclonals or monoclonal antibodies, mouses, mouse monoclonals
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Host
mouse, Murine monoclonal antibodies or Mabs are often conjugated and the isotype is IgG.
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Isotype
Mouse IgG2b
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Label
FITC
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Species
Mouse, Mouses
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Alternative name
Mouse Anti Mouse H2Db Antibody-FITC
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Alternative technique
antibodies, murine, fluorescine
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MeSH Data
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Name
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Concept
Scope note:
Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
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Tree numbers
- E05.196.401.143
- E05.301.300.096
- E05.478.566.320.200
- E05.601.262
- E05.601.470.320.200
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Qualifiers
ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data
Product images
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