Mouse-CD4/CD8 Human Antibody [HP2/6, 143-44]-FITC/PE
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Catalog number
BD-4F18PE1-50T
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Price
Please ask
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Size
50 TEST
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Description
Mouse-CD4/CD8 Human Antibody [HP2/6, 143-44]-FITC/PE is a human antibody resulting from the combination of two monoclonal antibodies CD4 and CD8, this reagent was [HP2/6] FITC conjugated and [143-44] PE conjugated, the product is reported for use in flow cytometric analysis for two color. this combined antibody reacts consecutively with both CD4 and CD8 antigen in mature helper and inducer lymphocytes, and in suppressor and cytotxic lymphocytes, used also to identify CD4+/CD8+ lymphocyte ratio.
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Conjugated
FITC/PE
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Clone
HP2/6, 143-44
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Application
Flow Cytomtry Method
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Genes name
CD4/CD8
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Formulatin
Phosphate buffred solution, 0,09% sodium Azide, may contain carrier protein-stabilizer
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Expiration
After 1 year
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Storage
2-8°C, store in dark, do not freeze
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Remark
For in Vitro Diagnostic Use.
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Properties
If you buy Antibodies supplied by Immunostep they should be stored frozen at - 24°C for long term storage and for short term at + 5°C. This Immunostep Fluorescein isothiocyanate (FITC) antibody is currently after some BD antibodies the most commonly used fluorescent dye for FACS. When excited at 488 nanometers, FITC has a green emission that's usually collected at 530 nanometers, the FL1 detector of a FACSCalibur or FACScan. FITC has a high quantum yield (efficiency of energy transfer from absorption to emission fluorescence) and approximately half of the absorbed photons are emitted as fluorescent light. For fluorescent microscopy applications, the 1 FITC is seldom used as it photo bleaches rather quickly though in flow cytometry applications, its photo bleaching effects are not observed due to a very brief interaction at the laser intercept. Immunostep FITC is highly sensitive to pH extremes. Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.
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Additional description
CD4+ T helper cell marker or cluster differentiator of white blood cells in FACS or flow cytometry. Used offer coupled to magnetic beads. GENTAUR makes custom magnetic bead couplings on 50 and 250 nm beads for certain clones. Cytotoxic T cells cluster differentiators for flow cytometry. This transmembrane glycoprotein is detected by monoclonals and has a lot of clones available with different affinity.
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Conjugation
Anti-FITC Antibody
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Test
Mouse or mice from the Mus musculus species are used for production of mouse monoclonal antibodies or mabs and as research model for humans in your lab. Mouse are mature after 40 days for females and 55 days for males. The female mice are pregnant only 20 days and can give birth to 10 litters of 6-8 mice a year. Transgenic, knock-out, congenic and inbread strains are known for C57BL/6, A/J, BALB/c, SCID while the CD-1 is outbred as strain.
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Latin name
Mus musculus
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French translation
anticorps
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Gene target
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Gene symbol
CD4, IGHVIII-44, CD8A, SNORD115-44, BOLA1, IGLV1-44, IGHVIV-44-1, IGHVII-44-2, ZNF141, MIR143
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Short name
Mouse-CD4/CD8 Antibody [HP2/6, 143-44]-FITC/PE
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Technique
Antibody, Mouse, FITC, antibodies against human proteins, antibodies for, Fluorescein, mouses
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Host
mouse
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Isotype
IgG2a, IgG2a
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Label
FITC
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Species
Human, Humans, Mouses
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Alternative name
Anti Human CD4/CD8 Antibody-FITC/PE
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Alternative technique
antibodies, murine, fluorescine
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Alternative to gene target
CD4 molecule, CD4mut, CD4 and IDBG-15034 and ENSG00000010610 and 920, protein homodimerization activity, Cell surfaces, Cd4 and IDBG-188351 and ENSMUSG00000023274 and 12504, CD4 and IDBG-640247 and ENSBTAG00000003255 and 407098
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Gene info
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Identity
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Gene
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Long gene name
CD4 molecule
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Synonyms gene name
- CD4 antigen (p55)
- T-cell surface glycoprotein CD4
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GenBank acession
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Locus
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Discovery year
1986-01-01
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Entrez gene record
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RefSeq identity
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Classification
- CD molecules
- C2-set domain containing
- V-set domain containing
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VEGA ID
Gene info
Gene info
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Identity
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Gene
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Long gene name
CD8a molecule
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Synonyms gene
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Synonyms gene name
- CD8 antigen, alpha polypeptide (p32)
- T-cell surface glycoprotein CD8 alpha chain
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Locus
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Discovery year
1986-01-01
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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Classification
- V-set domain containing
- CD molecules
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VEGA ID
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Locus Specific Databases
Gene info
Gene info
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Identity
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Gene
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Long gene name
bolA family member 1
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Synonyms gene name
- bolA-like 1 (E. coli)
- bolA homolog 1 (E. coli)
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Synonyms
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GenBank acession
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Locus
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Discovery year
2005-05-09
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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VEGA ID
Gene info
Gene info
Gene info
Gene info
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Identity
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Gene
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Long gene name
zinc finger protein 141
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Synonyms gene
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Synonyms gene name
- zinc finger protein 141 (clone pHZ-44)
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Synonyms
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GenBank acession
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Locus
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Discovery year
1993-02-11
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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Classification
- Zinc fingers C2H2-type
- MicroRNA protein coding host genes
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VEGA ID
Gene info
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Identity
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Gene
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Long gene name
microRNA 143
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Synonyms gene
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Synonyms
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Locus
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Discovery year
2004-04-23
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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Classification
MeSH Data
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Name
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Concept
Scope note:
Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
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Tree numbers
- E05.196.401.143
- E05.301.300.096
- E05.478.566.320.200
- E05.601.262
- E05.601.470.320.200
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Qualifiers
ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data
Product images
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