Mouse CD45.2 Mouse Monoclonal Antibody [104]-FITC
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Catalog number
BD-MO452F (V500)
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Price
Please ask
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Size
0,5 mg
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Description
Mouse CD45.2 Mouse Monoclonal Antibody [104]-FITC is a mouse monoclonal antibody, specific for [104] clones, this reagent was conjugated with FITC format and reported for use in flow cytometric analysis to identify CD45.1 antigens in all hematopoietic cells but not in mature erythrocytes and platelets, CD45.1 plays a role of T and B cells activation, phosphatase and signal transduction.
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Clone
104
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Conjugate
FITC
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Gene Name
CD45.2
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Other Names of gene
Ly-5.2, LCA
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Concentration
500 µg/mL
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Application
Flow Cytometry Method
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Immunogen
B10.S mouse thymocytes and splenocytes
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Formulation
Aqueous buffered solution containing protein stabilizer, and ≤0.09% sodium azide (NaN3).
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Storage
2-8°C, store in dark, do not freeze
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Expiration
After 1 year
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Remark
For Recherche Use Only.
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Properties
If you buy Antibodies supplied by Immunostep they should be stored frozen at - 24°C for long term storage and for short term at + 5°C. This Immunostep Fluorescein isothiocyanate (FITC) antibody is currently after some BD antibodies the most commonly used fluorescent dye for FACS. When excited at 488 nanometers, FITC has a green emission that's usually collected at 530 nanometers, the FL1 detector of a FACSCalibur or FACScan. FITC has a high quantum yield (efficiency of energy transfer from absorption to emission fluorescence) and approximately half of the absorbed photons are emitted as fluorescent light. For fluorescent microscopy applications, the 1 FITC is seldom used as it photo bleaches rather quickly though in flow cytometry applications, its photo bleaching effects are not observed due to a very brief interaction at the laser intercept. Immunostep FITC is highly sensitive to pH extremes.
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Conjugation
Anti-FITC Antibody
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About
Monoclonals of this antigen are available in different clones. Each murine monoclonal anibody has his own affinity specific for the clone. Mouse monoclonal antibodies are purified protein A or G and can be conjugated to FITC for flow cytometry or FACS and can be of different isotypes.
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Test
Mouse or mice from the Mus musculus species are used for production of mouse monoclonal antibodies or mabs and as research model for humans in your lab. Mouse are mature after 40 days for females and 55 days for males. The female mice are pregnant only 20 days and can give birth to 10 litters of 6-8 mice a year. Transgenic, knock-out, congenic and inbread strains are known for C57BL/6, A/J, BALB/c, SCID while the CD-1 is outbred as strain.
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Latin name
Mus musculus
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French translation
anticorps
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Gene target
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Gene symbol
PTPRCAP, PTPRC
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Short name
Mouse CD45 2 Mouse Monoclonal Antibody [104]-FITC
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Technique
Mouse monoclonal, Antibody, Mouse, FITC, antibodies against human proteins, antibodies for, Fluorescein, Monoclonals or monoclonal antibodies, mouses, mouse monoclonals
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Host
mouse, Murine monoclonal antibodies or Mabs are often conjugated and the isotype is IgG.
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Isotype
Mouse IgG2a
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Label
FITC
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Species
Mouse, Mouses
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Alternative name
Mouse Anti Mouse CD45.2 Antibody-FITC
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Alternative technique
antibodies, murine, fluorescine
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Gene info
Gene info
MeSH Data
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Name
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Concept
Scope note:
The number of CD4-POSITIVE T-LYMPHOCYTES per unit volume of BLOOD. Determination requires the use of a fluorescence-activated flow cytometer.
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Tree numbers
- E01.370.225.500.195.107.595.500.150
- E01.370.225.625.107.595.500.150
- E05.200.500.195.107.595.500.150
- E05.200.625.107.595.500.150
- E05.242.195.107.595.500.150
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Qualifiers
ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data
Product images
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