Mouse CD45.1 Mouse Monoclonal Antibody [A20]-Biotin
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Catalog number
BD-MO451B (V500)
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Price
Please ask
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Size
0,5 mg
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Description
Mouse CD45.1 Mouse Monoclonal Antibody [A20]-Biotin is a mouse monoclonal antibody, specific for [A20] clones, this reagent was conjugated with Biotin format and reported for use in flow cytometric analysis to identify CD45.1 antigens in all hematopoietic cells but not in mature erythrocytes and platelets, CD45.1 plays a role of T and B cells activation, phosphatase and signal transduction.
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Clone
A20
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Conjugate
Biotin
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Gene Name
CD45.1
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Other Names of gene
T200, Ly-5.1, LCA
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Concentration
500 µg/mL
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Application
Flow Cytometry Method
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Immunogen
SJL mouse thymocytes and splenocytes
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Formulation
Aqueous buffered solution containing protein stabilizer, and ≤0.09% sodium azide (NaN3).
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Storage
2-8°C, store in dark, do not freeze
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Expiration
After 1 year
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Remark
For Recherche Use Only.
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Properties
If you buy Antibodies supplied by Immunostep they should be stored frozen at - 24°C for long term storage and for short term at + 5°C. Biotin conjugates can be detected by horseradish peroxidase, alkaline phosphatase substrates or anti biotin conjugated antibodies. Avidin and Streptavidin bind to the small biotin and are couple to HRP or AP for ELISA. To break the streptavidin Biotin bond we suggest to use a 6 molar guanidine HCl solution with acidity of pH 1.6.
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Conjugation
Biotinylated
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About
Monoclonals of this antigen are available in different clones. Each murine monoclonal anibody has his own affinity specific for the clone. Mouse monoclonal antibodies are purified protein A or G and can be conjugated to FITC for flow cytometry or FACS and can be of different isotypes.
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Test
Mouse or mice from the Mus musculus species are used for production of mouse monoclonal antibodies or mabs and as research model for humans in your lab. Mouse are mature after 40 days for females and 55 days for males. The female mice are pregnant only 20 days and can give birth to 10 litters of 6-8 mice a year. Transgenic, knock-out, congenic and inbread strains are known for C57BL/6, A/J, BALB/c, SCID while the CD-1 is outbred as strain.
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Latin name
Mus musculus
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French translation
anticorps
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Gene target
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Gene symbol
TNFAIP3, IGKV1-27, PTPRCAP, PTPRC
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Short name
Mouse CD45 1 Mouse Monoclonal Antibody [A20]-
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Technique
Mouse monoclonal, Antibody, Mouse, antibodies against human proteins, antibodies for, Monoclonals or monoclonal antibodies, mouses, mouse monoclonals
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Host
mouse, Murine monoclonal antibodies or Mabs are often conjugated and the isotype is IgG.
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Isotype
Mouse IgG2a
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Label
Biotin
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Species
Mouse, Mouses
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Alternative name
Mouse Anti Mouse CD45.1 Antibody-Biotin
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Alternative technique
antibodies, murine
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Gene info
Gene info
Gene info
Gene info
MeSH Data
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Name
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Concept
Scope note:
Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
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Tree numbers
- E05.196.401.143
- E05.301.300.096
- E05.478.566.320.200
- E05.601.262
- E05.601.470.320.200
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Qualifiers
ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data
Product images
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