KAPPA/LAMDA/CD19 Human Antibody [polyclonal/polyclonal/J3.119]-FITC/PE/PE-Cyanine5
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Description
KAPPA/LAMDA/CD19 Human Antibody [polyclonal/polyclonal/J3.119]-FITC/PE/PE-Cyanine5 is a human antibody resulting from the mixture of three monclonal antibodies, this reagent was KAPPA POLYCLONAL FITC conjugated, LAMBDA POLYCLONAL PE conjugated and [J3.119] PE-Cyannine conjugated, the product is reported for use in flow cytometric analysis for three color, this antibody reacts with antigen of KAPPA, LAMBDA, and CD19 to identify B lymphocytes.
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Conjugated
FITC/ PE/ PE-Cyanine5
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Clone
polyclonal/polyclonal/J3.119
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Application
Flow Cytomtry Method
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Genes name
KAPPA/LAMBDA/CD19
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Formulatin
Phosphate buffred solution, 0,09% sodium Azide, may contain carrier protein-stabilizer
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Expiration
After 1 year
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Storage
2-8°C, store in dark, do not freeze
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Remark
For Recherche Use Only.
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Properties
If you buy Antibodies supplied by Immunostep they should be stored frozen at - 24°C for long term storage and for short term at + 5°C. This Immunostep Fluorescein isothiocyanate (FITC) antibody is currently after some BD antibodies the most commonly used fluorescent dye for FACS. When excited at 488 nanometers, FITC has a green emission that's usually collected at 530 nanometers, the FL1 detector of a FACSCalibur or FACScan. FITC has a high quantum yield (efficiency of energy transfer from absorption to emission fluorescence) and approximately half of the absorbed photons are emitted as fluorescent light. For fluorescent microscopy applications, the 1 FITC is seldom used as it photo bleaches rather quickly though in flow cytometry applications, its photo bleaching effects are not observed due to a very brief interaction at the laser intercept. Immunostep FITC is highly sensitive to pH extremes. Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.
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Conjugation
Anti-FITC Antibody
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Group
Polyclonals and antibodies
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About
Polyclonals can be used for Western blot, immunohistochemistry on frozen slices or parrafin fixed tissues. The advantage is that there are more epitopes available in a polyclonal antiserum to detect the proteins than in monoclonal sera.
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French translation
anticorps
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Gene target
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Gene symbol
ETV3, ERF, CD19, PTPRK, CDC42BPG
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Short name
KAPPA/LAMDA/CD19 Antibody [polyclonal/polyclonal/J3 119]-FITC/PE/PE-Cyanine5
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Technique
Polyclonal, Antibody, FITC, antibodies against human proteins, antibodies for, Fluorescein, Polyclonal antibodies (pAbs) are mostly rabbit or goat antibodies that are secreted by different B cells, whereas monoclonal antibodies come from a single N cell lineage. Pabs are a collection of immunoglobulin molecules that react against a specific antigen, each identifying a different epitope.
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Isotype
Rabbit polyclonal/Rabbit polyclonal/Mouse IgG
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Label
FITC
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Species
Human, Humans
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Alternative name
Anti Human KAPPA/LAMBDA/CD19 Antibody-FITC/ PE/ PE-Cyanine5
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Alternative technique
polyclonals, antibodies, fluorescine
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Alternative to gene target
CD19 molecule, B4 and CVID3, CD19 and IDBG-23791 and ENSG00000177455 and 930, protein binding, Cell surfaces, Cd19 and IDBG-209475 and ENSMUSG00000030724 and 12478, CD19 and IDBG-638894 and ENSBTAG00000032122 and
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Gene info
Gene info
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Identity
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Gene
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Long gene name
ETS2 repressor factor
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Synonyms gene name
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Synonyms
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GenBank acession
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Locus
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Discovery year
1998-07-17
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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Classification
- ETS transcription factor family
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VEGA ID
Gene info
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Identity
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Gene
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Long gene name
CD19 molecule
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Synonyms gene name
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Locus
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Discovery year
1991-06-04
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Entrez gene record
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Classification
- CD molecules
- Immunoglobulin like domain containing
- Minor histocompatibility antigens
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VEGA ID
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Locus Specific Databases
Gene info
Gene info
MeSH Data
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Name
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Concept
Scope note:
Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
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Tree numbers
- E05.196.401.143
- E05.301.300.096
- E05.478.566.320.200
- E05.601.262
- E05.601.470.320.200
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Qualifiers
ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data
Product images
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