Armenian Hamster CD154 Mouse Monoclonal Antibody [MR1]-FITC
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Catalog number
BD-MO154F(V25)
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Price
Please ask
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Size
0,025 mg
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Description
Armenian Hamster CD154 Mouse Monoclonal Antibody [MR1]-FITC is a mouse monoclonal antibody, specific for [MR1] clones, this product was conjugated with FITC format and reported for use in flow cytometric analysis to interact with CD154 in activated CD4+ T cells, CD154's function is the costimulation of T-B cells, this antibody inhibits T and B lymphocytes activation in vitro, and blocks lymphocyte responses to specific antigen in vivo.
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Clone
MR1
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Conjugate
FITC
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Gene Name
CD154
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Other Names of gene
CD40 ligand (CD40L), gp39, T-BAM, TRAP, Ly-62, TNFSF5
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Concentration
500 µg/mL
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Application
Flow Cytometry Method
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Immunogen
Activated mouse Th1 clone D1.10
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Formulation
Aqueous buffered solution containing protein stabilizer, and ≤0.09% sodium azide (NaN3).
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Storage
2-8°C, store in dark, do not freeze
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Expiration
After 1 year
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Remark
For Recherche Use Only.
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Properties
If you buy Antibodies supplied by Immunostep they should be stored frozen at - 24°C for long term storage and for short term at + 5°C. This Immunostep Fluorescein isothiocyanate (FITC) antibody is currently after some BD antibodies the most commonly used fluorescent dye for FACS. When excited at 488 nanometers, FITC has a green emission that's usually collected at 530 nanometers, the FL1 detector of a FACSCalibur or FACScan. FITC has a high quantum yield (efficiency of energy transfer from absorption to emission fluorescence) and approximately half of the absorbed photons are emitted as fluorescent light. For fluorescent microscopy applications, the 1 FITC is seldom used as it photo bleaches rather quickly though in flow cytometry applications, its photo bleaching effects are not observed due to a very brief interaction at the laser intercept. Immunostep FITC is highly sensitive to pH extremes. Armenian of Syrian hamster monoclonal IgGs are useful antibodies for double staining your IHC samples. Using mabs or monoclonal antibodies has a lot of advantages for Flow cytometry.
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Conjugation
Anti-FITC Antibody
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About
Monoclonals of this antigen are available in different clones. Each murine monoclonal anibody has his own affinity specific for the clone. Mouse monoclonal antibodies are purified protein A or G and can be conjugated to FITC for flow cytometry or FACS and can be of different isotypes.
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Test
Mouse or mice from the Mus musculus species are used for production of mouse monoclonal antibodies or mabs and as research model for humans in your lab. Mouse are mature after 40 days for females and 55 days for males. The female mice are pregnant only 20 days and can give birth to 10 litters of 6-8 mice a year. Transgenic, knock-out, congenic and inbread strains are known for C57BL/6, A/J, BALB/c, SCID while the CD-1 is outbred as strain.
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Latin name
Mus musculus
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French translation
anticorps
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Gene target
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Gene symbol
MR1, CD40LG
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Short name
Armenian CD154 Mouse Monoclonal Antibody [MR1]-FITC
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Technique
Mouse monoclonal, Antibody, Mouse, FITC, antibodies against human proteins, antibodies for, Fluorescein, Monoclonals or monoclonal antibodies, mouses, mouse monoclonals
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Host
Hamster, Murine monoclonal antibodies or Mabs are often conjugated and the isotype is IgG.
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Isotype
Armenian Hamster IgG
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Label
FITC
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Species
Mouse, Hamsters, Mouses
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Alternative name
Armenian Hamster Anti Mouse CD154 Antibody-FITC
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Alternative technique
antibodies, murine, fluorescine
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Alternative to gene target
major histocompatibility complex, class I-related, HLALS, MR1 and IDBG-105193 and ENSG00000153029 and 3140, peptide antigen binding, Extracellular, Mr1 and IDBG-199690 and ENSMUSG00000026471 and 15064, MR1 and IDBG-636942 and ENSBTAG00000009924 and 506206
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Gene info
Gene info
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Identity
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Gene
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Long gene name
CD40 ligand
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Synonyms gene
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Synonyms gene name
- tumor necrosis factor (ligand) superfamily, member 5 (hyper-IgM syndrome)
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Synonyms
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Synonyms name
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GenBank acession
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Locus
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Discovery year
1989-06-30
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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Classification
- CD molecules
- Tumor necrosis factor superfamily
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VEGA ID
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Locus Specific Databases
MeSH Data
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Name
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Concept
Scope note:
Test for tissue antigen using either a direct method, by conjugation of antibody with fluorescent dye (FLUORESCENT ANTIBODY TECHNIQUE, DIRECT) or an indirect method, by formation of antigen-antibody complex which is then labeled with fluorescein-conjugated anti-immunoglobulin antibody (FLUORESCENT ANTIBODY TECHNIQUE, INDIRECT). The tissue is then examined by fluorescence microscopy.
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Tree numbers
- E01.370.225.500.607.512.240
- E01.370.225.750.551.512.240
- E05.200.500.607.512.240
- E05.200.750.551.512.240
- E05.478.583.375
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Qualifiers
ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data
Product images
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