anti-V5 Host: Rabbit FITC Conjugated
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Catalog numberRV5-45F
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Price:
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Size1.0 mg
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DescriptionThis antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided. Antibody for research use. Most polyclonal antibodies pabs are raised in rabbits as rabbit polyclonal antibodies.
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PropertiesThis icl Fluorescein isothiocyanate (FITC) antibody is currently after some BD antibodies the most commonly used fluorescent dye for FACS. When excited at 488 nanometers, FITC has a green emission that's usually collected at 530 nanometers, the FL1 detector of a FACSCalibur or FACScan. FITC has a high quantum yield (efficiency of energy transfer from absorption to emission fluorescence) and approximately half of the absorbed photons are emitted as fluorescent light. For fluorescent microscopy applications, the 1 FITC is seldom used as it photo bleaches rather quickly though in flow cytometry applications, its photo bleaching effects are not observed due to a very brief interaction at the laser intercept. icl FITC is highly sensitive to pH extremes.
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ConjugationAnti-FITC Antibody
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AboutRabbits are used for polyclonal antibody production by icl. Rabbit antibodies are very stable and can be stored for several days at room temperature. icl adds sodium azide and glycerol to enhance the stability of the rabbit polyclonal antibodies. Anti-human, anti mouse antibodies to highly immunogenic selected peptide sequences are" monoclonal like" since the epitope to which they are directed is less than 35 amino acids long.
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Latin nameOryctolagus cuniculus
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Gene target
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Short nameanti-V5 : Rabbit FITC Conjugated
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TechniqueRabbit, anti-, anti, FITC, antibody to, antibodies, antibody Conjugates, Fluorescein
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HostRabbit, Rabbits
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LabelFITC
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Alternative nameAntibody toV5 production species: production species: rabbit fluorecein coupled
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Alternative techniquerabbit-anti, antibodies, fluorescine
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MeSH Data
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Name
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ConceptScope note: Test for tissue antigen using either a direct method, by conjugation of antibody with fluorescent dye (FLUORESCENT ANTIBODY TECHNIQUE, DIRECT) or an indirect method, by formation of antigen-antibody complex which is then labeled with fluorescein-conjugated anti-immunoglobulin antibody (FLUORESCENT ANTIBODY TECHNIQUE, INDIRECT). The tissue is then examined by fluorescence microscopy.
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Tree numbers
- E01.370.225.500.607.512.240
- E01.370.225.750.551.512.240
- E05.200.500.607.512.240
- E05.200.750.551.512.240
- E05.478.583.375
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Qualifiersethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data