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Description
Safranine, 0.5% w/v is a ready made staining solution reported to be used for Gram's counterstain .
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Application
For microbiological / pathological laboratories, hospitals, research settings and educational institutes. These stains augment tissue morphology, demonstrate important cellular components, aid in the evaluation of diseases, and identifies pathogenic organisms.
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Classification
Gram Stains
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Composition
Safranine O 0.500 gm Ethyl alcohol, 95% 100.000 ml
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Appearance
Red coloured solution.
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Clarity
Clear without any particles.
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Microscopic Examination
Gram staining is carried out where Safranin is used as counterstain and staining characteristic of organisms are observed under microscope by using oil immersion lens.
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Results
Gram-positive microorganisms : violet , Gram-negative microorganims : pinkish red.
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Storage
Store below 30°C in tightly closed container and away from bright light.
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Shelf Life
Use before expiry date on label.
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