RPMI-1640 w/ 2mM L-Glutamine, 10mM HEPES buffer and 4.5gms Glucose per litre

  • Catalog number
    AT223-5LX8
  • Price
    Please ask
  • Size
    8X5L
  • Description
    RPMI-1640 With 2mM L-Glutamine,10mM HEPES buffer and 4.5gms Glucose per litre Without Sodium pyruvate and Sodium bicarbonate Produced by HiMedia Laboratories is a nutrient blend of inorganic salts, organic compounds, amino acids, vitamins and carbohydrates.
  • Storage conditions
    Store at 2 - 8°C
  • Appearance
    Homogeneous powder
  • Solubility
    NA
  • Use
    used in laboratory chemicals, Manufacture of substances
  • Technical data
    Please contact us to request the technical data
  • Additional description
    Buffering solutions are useful to keep the pH range sable when using this reagent of Himedia. The Liter or liter is a metric system unit of volume equal to 1 cubic decimeter (dm3), 1,000 cubic centimeters (cm3) or 1/1,000 cubic meter. A cubic decimeter (or liter) occupies a volume of 10×10×10 centimeters (see figure) and is thus equal to one-thousandth of a cubic meter. Water baths of various volumes are available for lab research.
  • Buffer
    25mM HEPES (4-(2-hydroxyethyl)-1-piperazineethanesulfonic acid ) pH7 solution buffers are a media with a zwitterion organic chemical buffering agent; one of the twenty Good's buffers. HEPES DMEM medium is widely used in cell culture, largely because it is better at maintaining physiological pH 7 tot 7,5 despite changes in carbon dioxide concentration (produced by cellular respiration) when compared to bicarbonate buffers, which are also commonly used in cell culture. The dissociation of water decreases with falling temperature, but the dissociation constants (pK) of many other buffers do not change much with temperature. HEPES, 25 mM in RPMI and DMEM is like water in that its dissociation decreases as the temperature decreases. This makes HEPES RPMI 25 milli-molar media with or without L-glutamine a more effective buffering agent for maintaining enzyme structure and function at low temperatures.
  • Gene target
    RPMI-1640   2mM   L-Glutamine   10mM   5gms   Glucose   per  
  • Short name
    RPMI-1640 w/ 2mM L-Glutamine, 10mM 4 5gms Glucose per
  • Alternative name
    RPMI-1640 w/ 2mM L-Glutamine, 10mM HEPES solution and 4.5gms Glucose per litre
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