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Description
ONPG Broth is used to differentiate microorganisms on the bas is of beta-galactosidase activity.
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Alternative Name
Ortho-nitrophenyl beta-D-galactopyranoside Broth
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Type
Dehydrated Culture Media
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Origin
Animal
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Classification
Bacterial
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Composition g L
Casein Peptone 7.500 Sodium Phosphate Dibasic 0.350 O-Nitrophenyl-b-D-galactopyranoside 1.500 Sodium chloride 3.750 Final pH ( at 25°C) 7.5±0.2
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Quantity required per litre
13,10 g
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Appearance
Cream to yellow homogeneous free flowing powder
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Colour and Clarity of prepared medium
Light amber coloured clear solution in tubes
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Cultural response
Cultural characteristics observed after an incubation at 35-37°C for 18-48 hours.
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Storage
Store dehydrated medium and the prepared medium at 2-8°C
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Shelf Life
Use before expiry date on the label.
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Properties
º C. The broth is a modified culture medium for culturing isolated or enriched bacteria, yeasts and micro organisms.The buffered pH and low salt content of these media broths will ensure a perfect growth of the pathogens to be detected. The broth or culture medium is supplied in 500gr and 5 x 500gr quntities by Himedia. The broth's MSDS certificate is supplied for appoval of our broth's batch quality. The screening and selective enrichment of these pathogens and bacteriacan also be obtained by other broths and selective culture media supplements.